Altered loyalties of neuronal markers in cultured slices of resected human brain tissue.

TY - JOUR

T1 - Altered loyalties of neuronal markers in cultured slices of resected human brain tissue.

AU - Verwer, R.W.H.

AU - Sluiter, A.A.

AU - Balesar, R.A.

AU - Baayen, J.C.

AU - Speijer, D.

AU - Idema, R.

AU - Swaab, D.F.

PY - 2016

Y1 - 2016

N2 - Organotypic cultures from normal neocortical tissue obtained at epilepsy surgery show a severe injury response. This response involves both neuronal degeneration and the proliferation of reactive cells. A salient feature of the reactive cells is the co-expression of microglial and astrocytic markers. Surprisingly, the reactive cells also began to express neuronal markers Tubulin βIII and MAP2 adding to the confusion about their origin. Concomitant with their appearance in reactive cells MAP2 and Tubulin βIII expression disappeared from neurons. While NeuN expression decreased significantly, it did not entirely disappear from many neurons. Moreover, it was not observed in reactive cells, showing that NeuN is a reliable marker of neurons.

AB - Organotypic cultures from normal neocortical tissue obtained at epilepsy surgery show a severe injury response. This response involves both neuronal degeneration and the proliferation of reactive cells. A salient feature of the reactive cells is the co-expression of microglial and astrocytic markers. Surprisingly, the reactive cells also began to express neuronal markers Tubulin βIII and MAP2 adding to the confusion about their origin. Concomitant with their appearance in reactive cells MAP2 and Tubulin βIII expression disappeared from neurons. While NeuN expression decreased significantly, it did not entirely disappear from many neurons. Moreover, it was not observed in reactive cells, showing that NeuN is a reliable marker of neurons.

U2 - 10.1111/bpa.12330

DO - 10.1111/bpa.12330

M3 - Article

SN - 1015-6305

VL - 26

SP - 523

EP - 532

JO - Brain Pathology

JF - Brain Pathology

ER -