TY - JOUR
T1 - Ammonium-Oxidation at Low Ph by a Chemolithotrophic Bacterium Belonging to the Genus Nitrosospira
AU - De Boer, W.
AU - Klein Gunnewiek, P.J.A.
AU - Laanbroek, H.J.
N1 - Reporting year: 1995
Metis note: 2036; CTE; TME ; ME file:///L:/Endnotedatabases/NIOOPUB/pdfs/Pdfs1995/DeBoer_ea_2036.pdf
PY - 1995
Y1 - 1995
N2 - Ammonium-oxidizing bacteria of the genus Nitrosospira have often been isolated from acid soils. However, the lower pH- limit for nitrifying activity of these bacteria in pure cultures or in mixed cultures with Nitrobacter sp. is much higher than ambient soil-pH. This study shows that the acid- sensitive, ammonium-oxidizing bacterium Nitrosospira strain AHB1, which has been isolated from an acid heathland soil, could be adapted to oxidize ammonium at pH 4 in the presence of the acid-tolerant nitrite-oxidizing bacterium Nitrobacter strain NHB1. Adaptation could be achieved in two different ways: (1) by immobilizing the bacteria in alginate beads; and (2) by exposing them to pH-fluctuations. Thus, Nitrosospira spp may be involved in the oxidation of ammonium at an ambient low soil-pH, even though they appear to be acid-sensitive after isolation. [KEYWORDS: Acid forest soil; autotrophic nitrification; atmospheric deposition; nitrifying bacteria; heath soil; gel beads; nitrogen; nitrobacter; nitrite; netherlands]
AB - Ammonium-oxidizing bacteria of the genus Nitrosospira have often been isolated from acid soils. However, the lower pH- limit for nitrifying activity of these bacteria in pure cultures or in mixed cultures with Nitrobacter sp. is much higher than ambient soil-pH. This study shows that the acid- sensitive, ammonium-oxidizing bacterium Nitrosospira strain AHB1, which has been isolated from an acid heathland soil, could be adapted to oxidize ammonium at pH 4 in the presence of the acid-tolerant nitrite-oxidizing bacterium Nitrobacter strain NHB1. Adaptation could be achieved in two different ways: (1) by immobilizing the bacteria in alginate beads; and (2) by exposing them to pH-fluctuations. Thus, Nitrosospira spp may be involved in the oxidation of ammonium at an ambient low soil-pH, even though they appear to be acid-sensitive after isolation. [KEYWORDS: Acid forest soil; autotrophic nitrification; atmospheric deposition; nitrifying bacteria; heath soil; gel beads; nitrogen; nitrobacter; nitrite; netherlands]
U2 - 10.1016/0038-0717(94)00157-V
DO - 10.1016/0038-0717(94)00157-V
M3 - Article
SN - 0038-0717
VL - 27
SP - 127
EP - 132
JO - Soil Biology & Biochemistry
JF - Soil Biology & Biochemistry
IS - 2
ER -