An organoid biobank for childhood kidney cancers that captures disease and tissue heterogeneity

Camilla Calandrini; Frans Schutgens; Rurika Oka; Thanasis Margaritis; Tito Candelli; Luka Mathijsen; Carola Ammerlaan; Ravian L van Ineveld; Sepide Derakhshan; Sanne de Haan; Emmy Dolman; Philip Lijnzaad; Lars Custers; Harry Begthel; Hindrik H D Kerstens; Lindy L Visser; Maarten Rookmaaker; Marianne Verhaar; Godelieve A M Tytgat; Patrick Kemmeren; Ronald R de Krijger; Reem Al-Saadi; Kathy Pritchard-Jones; Marcel Kool; Anne C Rios; Marry M van den Heuvel-Eibrink; Jan J Molenaar; Ruben van Boxtel; Frank C P Holstege; Hans Clevers; Jarno Drost

doi:10.1038/s41467-020-15155-6

An organoid biobank for childhood kidney cancers that captures disease and tissue heterogeneity

Camilla Calandrini, Frans Schutgens, Rurika Oka, Thanasis Margaritis, Tito Candelli, Luka Mathijsen, Carola Ammerlaan, Ravian L van Ineveld, Sepide Derakhshan, Sanne de Haan, Emmy Dolman, Philip Lijnzaad, Lars Custers, Harry Begthel, Hindrik H D Kerstens, Lindy L Visser, Maarten Rookmaaker, Marianne Verhaar, Godelieve A M Tytgat, Patrick KemmerenRonald R de Krijger, Reem Al-Saadi, Kathy Pritchard-Jones, Marcel Kool, Anne C Rios, Marry M van den Heuvel-Eibrink, Jan J Molenaar, Ruben van Boxtel, Frank C P Holstege, Hans Clevers, Jarno Drost

Hubrecht Institute

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

196 Citaten (Scopus)

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Kidney tumours are among the most common solid tumours in children, comprising distinct subtypes differing in many aspects, including cell-of-origin, genetics, and pathology. Pre-clinical cell models capturing the disease heterogeneity are currently lacking. Here, we describe the first paediatric cancer organoid biobank. It contains tumour and matching normal kidney organoids from over 50 children with different subtypes of kidney cancer, including Wilms tumours, malignant rhabdoid tumours, renal cell carcinomas, and congenital mesoblastic nephromas. Paediatric kidney tumour organoids retain key properties of native tumours, useful for revealing patient-specific drug sensitivities. Using single cell RNA-sequencing and high resolution 3D imaging, we further demonstrate that organoid cultures derived from Wilms tumours consist of multiple different cell types, including epithelial, stromal and blastemal-like cells. Our organoid biobank captures the heterogeneity of paediatric kidney tumours, providing a representative collection of well-characterised models for basic cancer research, drug-screening and personalised medicine.

Originele taal-2	Engels
Pagina's (van-tot)	1310
Tijdschrift	Nature Communications
Volume	11
Nummer van het tijdschrift	1
DOI's	https://doi.org/10.1038/s41467-020-15155-6
Status	Gepubliceerd - 11 mrt. 2020

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Calandrini, C., Schutgens, F., Oka, R., Margaritis, T., Candelli, T., Mathijsen, L., Ammerlaan, C., van Ineveld, R. L., Derakhshan, S., de Haan, S., Dolman, E., Lijnzaad, P., Custers, L., Begthel, H., Kerstens, H. H. D., Visser, L. L., Rookmaaker, M., Verhaar, M., Tytgat, G. A. M., ... Drost, J. (2020). An organoid biobank for childhood kidney cancers that captures disease and tissue heterogeneity. Nature Communications, 11(1), 1310. https://doi.org/10.1038/s41467-020-15155-6

@article{e9ca4aea8be64568a0e88d45b2ee947f,

title = "An organoid biobank for childhood kidney cancers that captures disease and tissue heterogeneity",

abstract = "Kidney tumours are among the most common solid tumours in children, comprising distinct subtypes differing in many aspects, including cell-of-origin, genetics, and pathology. Pre-clinical cell models capturing the disease heterogeneity are currently lacking. Here, we describe the first paediatric cancer organoid biobank. It contains tumour and matching normal kidney organoids from over 50 children with different subtypes of kidney cancer, including Wilms tumours, malignant rhabdoid tumours, renal cell carcinomas, and congenital mesoblastic nephromas. Paediatric kidney tumour organoids retain key properties of native tumours, useful for revealing patient-specific drug sensitivities. Using single cell RNA-sequencing and high resolution 3D imaging, we further demonstrate that organoid cultures derived from Wilms tumours consist of multiple different cell types, including epithelial, stromal and blastemal-like cells. Our organoid biobank captures the heterogeneity of paediatric kidney tumours, providing a representative collection of well-characterised models for basic cancer research, drug-screening and personalised medicine.",

author = "Camilla Calandrini and Frans Schutgens and Rurika Oka and Thanasis Margaritis and Tito Candelli and Luka Mathijsen and Carola Ammerlaan and {van Ineveld}, {Ravian L} and Sepide Derakhshan and {de Haan}, Sanne and Emmy Dolman and Philip Lijnzaad and Lars Custers and Harry Begthel and Kerstens, {Hindrik H D} and Visser, {Lindy L} and Maarten Rookmaaker and Marianne Verhaar and Tytgat, {Godelieve A M} and Patrick Kemmeren and {de Krijger}, {Ronald R} and Reem Al-Saadi and Kathy Pritchard-Jones and Marcel Kool and Rios, {Anne C} and {van den Heuvel-Eibrink}, {Marry M} and Molenaar, {Jan J} and {van Boxtel}, Ruben and Holstege, {Frank C P} and Hans Clevers and Jarno Drost",

year = "2020",

month = mar,

day = "11",

doi = "10.1038/s41467-020-15155-6",

language = "English",

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journal = "Nature Communications",

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Calandrini, C, Schutgens, F, Oka, R, Margaritis, T, Candelli, T, Mathijsen, L, Ammerlaan, C, van Ineveld, RL, Derakhshan, S, de Haan, S, Dolman, E, Lijnzaad, P, Custers, L, Begthel, H, Kerstens, HHD, Visser, LL, Rookmaaker, M, Verhaar, M, Tytgat, GAM, Kemmeren, P, de Krijger, RR, Al-Saadi, R, Pritchard-Jones, K, Kool, M, Rios, AC, van den Heuvel-Eibrink, MM, Molenaar, JJ, van Boxtel, R, Holstege, FCP, Clevers, H & Drost, J 2020, 'An organoid biobank for childhood kidney cancers that captures disease and tissue heterogeneity', Nature Communications, vol. 11, nr. 1, blz. 1310. https://doi.org/10.1038/s41467-020-15155-6

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AU - Calandrini, Camilla

AU - Schutgens, Frans

AU - Oka, Rurika

AU - Margaritis, Thanasis

AU - Candelli, Tito

AU - Mathijsen, Luka

AU - Ammerlaan, Carola

AU - van Ineveld, Ravian L

AU - Derakhshan, Sepide

AU - de Haan, Sanne

AU - Dolman, Emmy

AU - Lijnzaad, Philip

AU - Custers, Lars

AU - Begthel, Harry

AU - Kerstens, Hindrik H D

AU - Visser, Lindy L

AU - Rookmaaker, Maarten

AU - Verhaar, Marianne

AU - Tytgat, Godelieve A M

AU - Kemmeren, Patrick

AU - de Krijger, Ronald R

AU - Al-Saadi, Reem

AU - Pritchard-Jones, Kathy

AU - Kool, Marcel

AU - Rios, Anne C

AU - van den Heuvel-Eibrink, Marry M

AU - Molenaar, Jan J

AU - van Boxtel, Ruben

AU - Holstege, Frank C P

AU - Clevers, Hans

AU - Drost, Jarno

PY - 2020/3/11

Y1 - 2020/3/11

N2 - Kidney tumours are among the most common solid tumours in children, comprising distinct subtypes differing in many aspects, including cell-of-origin, genetics, and pathology. Pre-clinical cell models capturing the disease heterogeneity are currently lacking. Here, we describe the first paediatric cancer organoid biobank. It contains tumour and matching normal kidney organoids from over 50 children with different subtypes of kidney cancer, including Wilms tumours, malignant rhabdoid tumours, renal cell carcinomas, and congenital mesoblastic nephromas. Paediatric kidney tumour organoids retain key properties of native tumours, useful for revealing patient-specific drug sensitivities. Using single cell RNA-sequencing and high resolution 3D imaging, we further demonstrate that organoid cultures derived from Wilms tumours consist of multiple different cell types, including epithelial, stromal and blastemal-like cells. Our organoid biobank captures the heterogeneity of paediatric kidney tumours, providing a representative collection of well-characterised models for basic cancer research, drug-screening and personalised medicine.

AB - Kidney tumours are among the most common solid tumours in children, comprising distinct subtypes differing in many aspects, including cell-of-origin, genetics, and pathology. Pre-clinical cell models capturing the disease heterogeneity are currently lacking. Here, we describe the first paediatric cancer organoid biobank. It contains tumour and matching normal kidney organoids from over 50 children with different subtypes of kidney cancer, including Wilms tumours, malignant rhabdoid tumours, renal cell carcinomas, and congenital mesoblastic nephromas. Paediatric kidney tumour organoids retain key properties of native tumours, useful for revealing patient-specific drug sensitivities. Using single cell RNA-sequencing and high resolution 3D imaging, we further demonstrate that organoid cultures derived from Wilms tumours consist of multiple different cell types, including epithelial, stromal and blastemal-like cells. Our organoid biobank captures the heterogeneity of paediatric kidney tumours, providing a representative collection of well-characterised models for basic cancer research, drug-screening and personalised medicine.

U2 - 10.1038/s41467-020-15155-6

DO - 10.1038/s41467-020-15155-6

M3 - Article

C2 - 32161258

SN - 2041-1723

VL - 11

SP - 1310

JO - Nature Communications

JF - Nature Communications

IS - 1

ER -

An organoid biobank for childhood kidney cancers that captures disease and tissue heterogeneity

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