Analyses of black fungi by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS): species-level identification of clinical isolates of Exophiala dermatitidis

Nahid Kondori, Marcel Erhard, Christina Welinder-Olsson, Marizeth Groenewald, G.J.M. Verkley, Edward R. B. Moore, Derek Jamieson (Redacteur)

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    Samenvatting

    Nahid Kondori1,*, Marcel Erhard2, Christina Welinder-Olsson1, Marizeth Groenewald3, Gerard Verkley3 and Edward R. B. Moore1,41Department of Infectious Diseases, Sahlgrenska Academy of the University of Gothenburg, SE-40234 Gothenburg, Sweden2RIPAC-LABOR GmbH, D-14476 Potsdam, Germany3Centraalbureau voor Schimmelcultures (CBS), CBS-KNAW Fungal Biodiversity Centre, 3584 CT Utrecht, Netherlands4Culture Collection University of Gothenburg, Sahlgrenska Academy of the University of Gothenburg, SE-40234 Gothenburg, Sweden↵*Corresponding author: Department of Infectious Diseases, University of Gothenburg, Box 7193, 40234 Gothenburg, Sweden. Tel: +46-31-342-4226; Fax: +46-31-342-4975; E-mail: nahid.kondori{at}microbio.gu.seRevision received July 8, 2014.Accepted November 3, 2014.AbstractConventional mycological identifications based on the recognition of morphological characteristics can be problematic. A relatively new methodology applicable for the identification of microorganisms is based on the exploitation of taxon- specific mass patterns recorded from abundant cell proteins directly from whole-cell preparations, using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). This study reports the application of MALDI-TOF MS for the differentiation and identifications of black yeasts, isolated from the respiratory tracts of patients with cystic fibrosis (CF). Initial phenotypic and DNA sequence-based analyses identified these isolates to be Exophiala dermatitidis. The type strains of E. dermatitidis (CBS 207.35T) and other species of Exophiala were included in the MALDI-TOF MS analyses to establish the references for comparing the mass spectra of the clinical isolates of Exophiala. MALDI-TOF MS analyses exhibited extremely close relationships among the clinical isolates and with the spectra generated from the type strain of E. dermatitidis. The relationships observed between the E. dermatitidis strains from the MALDI-TOF MS profiling analyses were supported by DNA sequence-based analyses of the rRNA ITS1 and ITS2 regions. These data demonstrated the applicability of MALDI-TOF MS as a reliable, rapid and cost-effective method for the identification of isolates of E. dermatitidis and other clinically relevant fungi and yeasts that typically are difficult to identify by conventional methods.Exophiala dermatitidisfungiMALDI-TOF MSwhole-cell protein profiling© FEMS 2014. All rights reserved. For permissions, please e-mail: journals.permissions{at}oup.com
    Originele taal-2Engels
    Pagina's (van-tot)1-6
    Aantal pagina's6
    TijdschriftFEMS Microbiology Letters
    Volume362
    Nummer van het tijdschrift1
    DOI's
    StatusGepubliceerd - 21 jan 2015

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