Applicability and Reliability of Gel-Filtration to Study Aquatic Humic Substances Revisited - the Effects of Ph on Molecular-Size Distributions

P.J. Shaw; H. De Haan; R.I. Jones

doi:10.1080/09593339409385481

Applicability and Reliability of Gel-Filtration to Study Aquatic Humic Substances Revisited - the Effects of Ph on Molecular-Size Distributions

P.J. Shaw, H. De Haan, R.I. Jones

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

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The effect of pH on the molecular size distribution of dissolved humic substances (DHS) in surface waters from four different lakes in northern Europe was studied using Sephadex G‐100 gel filtration chromatography (GFC). New methods for the analysis of the elution profiles obtained are introduced, employing parallel graphical and statistical approaches. For all samples investigated, the proportion of the DHS eluted in the excluded fraction tended to diminish with decreasing pH. At the same time, the remaining DHS eluted tended to decrease in apparent molecular size with decreasing pH. Analyses of the elution profiles revealed that the apparent conformational flexibility of the DHS in the samples was greatest for the samples of lowest ionic strength. The applicability and reliability of GFC as a tool for the investigation of aquatic humic substances are discussed.

Originele taal-2	Engels
Pagina's (van-tot)	753-764
Tijdschrift	Environmental technology
Volume	15
Nummer van het tijdschrift	8
DOI's	https://doi.org/10.1080/09593339409385481
Status	Gepubliceerd - 1994

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10.1080/09593339409385481

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@article{7b64fe9c6afc4a5893f3afb383f7d78b,

title = "Applicability and Reliability of Gel-Filtration to Study Aquatic Humic Substances Revisited - the Effects of Ph on Molecular-Size Distributions",

abstract = "The effect of pH on the molecular size distribution of dissolved humic substances (DHS) in surface waters from four different lakes in northern Europe was studied using Sephadex G‐100 gel filtration chromatography (GFC). New methods for the analysis of the elution profiles obtained are introduced, employing parallel graphical and statistical approaches. For all samples investigated, the proportion of the DHS eluted in the excluded fraction tended to diminish with decreasing pH. At the same time, the remaining DHS eluted tended to decrease in apparent molecular size with decreasing pH. Analyses of the elution profiles revealed that the apparent conformational flexibility of the DHS in the samples was greatest for the samples of lowest ionic strength. The applicability and reliability of GFC as a tool for the investigation of aquatic humic substances are discussed.",

author = "P.J. Shaw and {De Haan}, H. and R.I. Jones",

note = "Reporting year: 1994 Metis note: Limnological Institute Publication no: 625",

year = "1994",

doi = "10.1080/09593339409385481",

language = "English",

volume = "15",

pages = "753--764",

journal = "Environmental Technology (United Kingdom)",

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}

Applicability and Reliability of Gel-Filtration to Study Aquatic Humic Substances Revisited - the Effects of Ph on Molecular-Size Distributions. / Shaw, P.J.; De Haan, H.; Jones, R.I.

In: Environmental technology, Vol. 15, Nr. 8, 1994, blz. 753-764.

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

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T1 - Applicability and Reliability of Gel-Filtration to Study Aquatic Humic Substances Revisited - the Effects of Ph on Molecular-Size Distributions

AU - Shaw, P.J.

AU - De Haan, H.

AU - Jones, R.I.

N1 - Reporting year: 1994 Metis note: Limnological Institute Publication no: 625

PY - 1994

Y1 - 1994

N2 - The effect of pH on the molecular size distribution of dissolved humic substances (DHS) in surface waters from four different lakes in northern Europe was studied using Sephadex G‐100 gel filtration chromatography (GFC). New methods for the analysis of the elution profiles obtained are introduced, employing parallel graphical and statistical approaches. For all samples investigated, the proportion of the DHS eluted in the excluded fraction tended to diminish with decreasing pH. At the same time, the remaining DHS eluted tended to decrease in apparent molecular size with decreasing pH. Analyses of the elution profiles revealed that the apparent conformational flexibility of the DHS in the samples was greatest for the samples of lowest ionic strength. The applicability and reliability of GFC as a tool for the investigation of aquatic humic substances are discussed.

AB - The effect of pH on the molecular size distribution of dissolved humic substances (DHS) in surface waters from four different lakes in northern Europe was studied using Sephadex G‐100 gel filtration chromatography (GFC). New methods for the analysis of the elution profiles obtained are introduced, employing parallel graphical and statistical approaches. For all samples investigated, the proportion of the DHS eluted in the excluded fraction tended to diminish with decreasing pH. At the same time, the remaining DHS eluted tended to decrease in apparent molecular size with decreasing pH. Analyses of the elution profiles revealed that the apparent conformational flexibility of the DHS in the samples was greatest for the samples of lowest ionic strength. The applicability and reliability of GFC as a tool for the investigation of aquatic humic substances are discussed.

U2 - 10.1080/09593339409385481

DO - 10.1080/09593339409385481

M3 - Article

SN - 0959-3330

VL - 15

SP - 753

EP - 764

JO - Environmental Technology (United Kingdom)

JF - Environmental Technology (United Kingdom)

IS - 8

ER -

Applicability and Reliability of Gel-Filtration to Study Aquatic Humic Substances Revisited - the Effects of Ph on Molecular-Size Distributions

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