TY - JOUR
T1 - AraR plays a more dominant role than XlnR in plant biomass conversion in Penicillium subrubescens
AU - Liu, Dujuan
AU - Xu, Li
AU - Peng, Mao
AU - Lipzen, Anna
AU - Ng, Vivian
AU - Savage, Emily
AU - Zhang, Yu
AU - Grigoriev, Igor V.
AU - Garrigues, Sandra
AU - de Vries, Ronald P.
N1 - Publisher Copyright:
© 2024 The Authors
PY - 2024/1
Y1 - 2024/1
N2 - Penicillium subrubescens is a promising candidate for industrial applications as its plant cell wall-degrading enzyme production levels and saccharification abilities are similar to that of the well-established industrial species Aspergillus niger. Interestingly, it has an expanded repertoire of hemicellulases, pectinases and inulinases in its genome compared to other Penicillia, that may enable a more targeted degradation of the corresponding polysaccharides. The transcriptional factor XlnR is essential for the expression of xylanolytic genes and is commonly found in genomes of filamentous ascomycete fungi. AraR (a homolog of XlnR) controls the arabinanolytic system as well as L-arabinose catabolism in Eurotiomycetes. In this study, we generated P. subrubescens ΔxlnR, ΔaraR and ΔxlnRΔaraR mutants and analyzed the transcriptional response of these strains to the monosaccharides D-xylose and L-arabinose, and the polysaccharide wheat arabinoxylan to identify the genes and pathways regulated by these TFs in P. subrubescens. Transcriptomic data revealed that AraR plays a more dominant role in plant biomass conversion in P. subrubescens than XlnR.
AB - Penicillium subrubescens is a promising candidate for industrial applications as its plant cell wall-degrading enzyme production levels and saccharification abilities are similar to that of the well-established industrial species Aspergillus niger. Interestingly, it has an expanded repertoire of hemicellulases, pectinases and inulinases in its genome compared to other Penicillia, that may enable a more targeted degradation of the corresponding polysaccharides. The transcriptional factor XlnR is essential for the expression of xylanolytic genes and is commonly found in genomes of filamentous ascomycete fungi. AraR (a homolog of XlnR) controls the arabinanolytic system as well as L-arabinose catabolism in Eurotiomycetes. In this study, we generated P. subrubescens ΔxlnR, ΔaraR and ΔxlnRΔaraR mutants and analyzed the transcriptional response of these strains to the monosaccharides D-xylose and L-arabinose, and the polysaccharide wheat arabinoxylan to identify the genes and pathways regulated by these TFs in P. subrubescens. Transcriptomic data revealed that AraR plays a more dominant role in plant biomass conversion in P. subrubescens than XlnR.
KW - Penicillium subrubescens
KW - Plant biomass degradation
KW - Transcription factors
KW - Transcriptomics
UR - http://www.scopus.com/inward/record.url?scp=85200841666&partnerID=8YFLogxK
U2 - 10.1016/j.crbiot.2024.100243
DO - 10.1016/j.crbiot.2024.100243
M3 - Article
AN - SCOPUS:85200841666
VL - 8
JO - Current Research in Biotechnology
JF - Current Research in Biotechnology
M1 - 100243
ER -