TY - JOUR
T1 - Aspergillus hubkae, a Novel Species Isolated from a Patient with Probable Invasive Pulmonary Aspergillosis
AU - Zhou, Ya Bin
AU - Rezaei-Matehkolaei, Ali
AU - Meijer, Martin
AU - Kraak, Bart
AU - Gerrits van den Ende, Bert
AU - Hagen, Ferry
AU - Afzalzadeh, Sara
AU - Kiasat, Neda
AU - Takesh, Ameneh
AU - Hoseinnejad, Akbar
AU - Houbraken, Jos
N1 - © 2024. The Author(s), under exclusive licence to Springer Nature B.V.
PY - 2024/5/11
Y1 - 2024/5/11
N2 - A 50-year-old man, previously diagnosed with pulmonary tuberculosis and lung cavities, presented with symptoms including fever, shortness of breath, and cough. A pulmonary CT scan revealed multiple cavities, consolidation and tree-in-bud in the upper lungs. Further investigation through direct examination of bronchoalveolar lavage fluid showed septate hyphae with dichotomous acute branching. Subsequent isolation and morphological analysis identified the fungus as belonging to Aspergillus section Nigri. The patient was diagnosed with probable invasive pulmonary aspergillosis and successfully treated with a three-month oral voriconazole therapy. Phylogenetic analysis based on partial β-tubulin, calmodulin and RNA polymerase second largest subunit sequences revealed that the isolate represents a putative new species related to Aspergillus brasiliensis, and is named Aspergillus hubkae here. Antifungal susceptibility testing demonstrated that the isolate is resistant to itraconazole but susceptible to voriconazole. This phenotypic and genetic characterization of A. hubkae, along with the associated case report, will serve as a valuable resource for future diagnoses of infections caused by this species. It will also contribute to more precise and effective patient management strategies in similar clinical scenarios.
AB - A 50-year-old man, previously diagnosed with pulmonary tuberculosis and lung cavities, presented with symptoms including fever, shortness of breath, and cough. A pulmonary CT scan revealed multiple cavities, consolidation and tree-in-bud in the upper lungs. Further investigation through direct examination of bronchoalveolar lavage fluid showed septate hyphae with dichotomous acute branching. Subsequent isolation and morphological analysis identified the fungus as belonging to Aspergillus section Nigri. The patient was diagnosed with probable invasive pulmonary aspergillosis and successfully treated with a three-month oral voriconazole therapy. Phylogenetic analysis based on partial β-tubulin, calmodulin and RNA polymerase second largest subunit sequences revealed that the isolate represents a putative new species related to Aspergillus brasiliensis, and is named Aspergillus hubkae here. Antifungal susceptibility testing demonstrated that the isolate is resistant to itraconazole but susceptible to voriconazole. This phenotypic and genetic characterization of A. hubkae, along with the associated case report, will serve as a valuable resource for future diagnoses of infections caused by this species. It will also contribute to more precise and effective patient management strategies in similar clinical scenarios.
KW - Humans
KW - Male
KW - Middle Aged
KW - Antifungal Agents/therapeutic use
KW - Aspergillus/isolation & purification
KW - Bronchoalveolar Lavage Fluid/microbiology
KW - Cluster Analysis
KW - DNA, Fungal/genetics
KW - Invasive Pulmonary Aspergillosis/microbiology
KW - Itraconazole/pharmacology
KW - Microbial Sensitivity Tests
KW - Microscopy
KW - Phylogeny
KW - Sequence Analysis, DNA
KW - Tomography, X-Ray Computed
KW - Treatment Outcome
KW - Tubulin/genetics
KW - Voriconazole/therapeutic use
U2 - 10.1007/s11046-024-00848-z
DO - 10.1007/s11046-024-00848-z
M3 - Article
C2 - 38734862
SN - 0301-486X
VL - 189
SP - 44
JO - Mycopathologia
JF - Mycopathologia
IS - 3
ER -