Aspergillus, Penicillium and Talaromyces isolated from house dust samples collected around the world

TY - JOUR

T1 - Aspergillus, Penicillium and Talaromyces isolated from house dust samples collected around the world

AU - Visagie, C M

AU - Hirooka, Y

AU - Tanney, J B

AU - Whitfield, E

AU - Mwange, K

AU - Meijer, M

AU - Amend, A S

AU - Seifert, K A

AU - Samson, R A

PY - 2014/6

Y1 - 2014/6

N2 - As part of a worldwide survey of the indoor mycobiota, dust was collected from nine countries. Analyses of dust samples included the culture-dependent dilution-to-extinction method and the culture-independent 454-pyrosequencing. Of the 7 904 isolates, 2 717 isolates were identified as belonging to Aspergillus, Penicillium and Talaromyces. The aim of this study was to identify isolates to species level and describe the new species found. Secondly, we wanted to create a reliable reference sequence database to be used for next-generation sequencing projects. Isolates represented 59 Aspergillus species, including eight undescribed species, 49 Penicillium species of which seven were undescribed and 18 Talaromyces species including three described here as new. In total, 568 ITS barcodes were generated, and 391 β-tubulin and 507 calmodulin sequences, which serve as alternative identification markers.

AB - As part of a worldwide survey of the indoor mycobiota, dust was collected from nine countries. Analyses of dust samples included the culture-dependent dilution-to-extinction method and the culture-independent 454-pyrosequencing. Of the 7 904 isolates, 2 717 isolates were identified as belonging to Aspergillus, Penicillium and Talaromyces. The aim of this study was to identify isolates to species level and describe the new species found. Secondly, we wanted to create a reliable reference sequence database to be used for next-generation sequencing projects. Isolates represented 59 Aspergillus species, including eight undescribed species, 49 Penicillium species of which seven were undescribed and 18 Talaromyces species including three described here as new. In total, 568 ITS barcodes were generated, and 391 β-tubulin and 507 calmodulin sequences, which serve as alternative identification markers.

U2 - 10.1016/j.simyco.2014.07.002

DO - 10.1016/j.simyco.2014.07.002

M3 - Article

C2 - 25492981

SN - 0166-0616

VL - 78

SP - 63

EP - 139

JO - Studies in Mycology

JF - Studies in Mycology

ER -