Aureobasidium melanogenum: a native of dark biofinishes on oil treated wood

Elke J. van Nieuwenhuijzen, Jos A M P Houbraken, Martin Meijer, Olaf C G Adan, Robert A Samson

    Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgaveArtikelWetenschappelijkpeer review

    Samenvatting

    The genus Aureobasidium, which is known as a wood staining mould, has been detected on oil treated woods in the specific stain formation called biofinish. This biofinish is used to develop a new protective, self-healing and decorative biotreatment for wood. In order to understand and control biofinish formation on oil treated wood, the occurrence of different Aureobasidium species on various wood surfaces was studied. Phenotypic variability within Aureobasidium strains presented limitations of morphological identification of Aureobasidium species. PCR amplification and Sanger sequencing of ITS and RPB2 were used to identify the culturable Aureobasidium species composition in mould stained wood surfaces with and without a biofinish. The analysed isolates showed that several Aureobasidium species were present and that Aureobasidium melanogenum was predominantly detected, regardless of the presence of a biofinish and the type of substrate. A. melanogenum was detected on wood samples exposed in the Netherlands, Cameroon, South Africa, Australia and Norway. ITS-specific PCR amplification, cloning and sequencing of DNA extracted from biofinish samples confirmed results of the culturing based method: A. melanogenum is predominant within the Aureobasidium population of biofinishes on pine sapwood treated with raw linseed oil and the outdoor placement in the Netherlands.

    Originele taal-2Engels
    Pagina's (van-tot)661-83
    Aantal pagina's23
    TijdschriftAntonie Van Leeuwenhoek International Journal of General and Molecular Microbiology
    Volume109
    Nummer van het tijdschrift5
    DOI's
    StatusGepubliceerd - mei 2016

    Vingerafdruk

    Duik in de onderzoeksthema's van 'Aureobasidium melanogenum: a native of dark biofinishes on oil treated wood'. Samen vormen ze een unieke vingerafdruk.

    Citeer dit