Beneficial microbial species and metabolites alleviate soybean oxidative damage and increase grain yield during short dry spells

Luiz Moretti de Souza; Carlos A. C. Crusciol; João William Bossolani; Juliano C. Calonego; Adônis Moreira; Ariani Garcia; L. Momesso; Eiko Kuramae; Mariangela Hungria

doi:10.1016/j.eja.2021.126293

Beneficial microbial species and metabolites alleviate soybean oxidative damage and increase grain yield during short dry spells

Luiz Moretti de Souza, Carlos A. C. Crusciol (Co-auteur), João William Bossolani, Juliano C. Calonego, Adônis Moreira, Ariani Garcia, L. Momesso, Eiko Kuramae (Co-auteur), Mariangela Hungria (Co-auteur)

NIOO - Microbial Ecology (ME)

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

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Short dry spells are an important grain yield constraint in tropical regions. Plant growth-promoting bacteria (PGPB) and their metabolites can mitigate the impact of drought stress by promoting changes in plant metabolism, physiology, and biochemistry. However, the effects of PGPB on soybean [Glycine max (L.) Merril] under drought stress in tropical regions have not been established. The experiments were carried out under tropical field conditions with short dry spells. Therefore, in this study we used a three-factorial trial to evaluate the effects of bacterial consortium consisting of N2-fixing Bradyrhizobium japonicum (strain SEMIA 5079) and Bradyrhizobium diazoefficiens (strain SEMIA 5080), the biocontrol agent Bacillus subtilis (strain QST 713), and the plant growth-promoting Azospirillum brasilense (strains Ab-V5 and Ab-V6) with or without application of microbial secondary metabolites (MSM, rhizobial metabolites enriched in lipo-chitooligosaccharides (LCOs)) during two growing seasons. Photosynthetic pigments, gas exchange parameters, antioxidant enzyme activity and proline concentrations in leaves, nodulation, plant growth development and grain yield were evaluated. The bacterial consortium comprising Bradyrhizobium spp., A. brasilense strains and MSM application increased the contents of chlorophyll a (14.5 %), chlorophyll b (30.8 %), total chlorophyll (17.2 %), and total carotenoids (27.3 %) compared with Bradyrhizobium spp. treatment alone. This consortium also increased the net photosynthetic rate (17.7 %), stomatal conductance (56.5 %), internal CO2 concentration in the substomatal chamber (8.3 %), and transpiration (44 %) compared with plants that received the standard inoculation (Bradyrhizobium spp. only), while reducing the leaf contents of hydrogen peroxide (−18.8 %) and proline (−29.4 %), lipid peroxidation (−15.9 %), and the activities of superoxide dismutase (−18.2 %), catalase (−21.2 %), and ascorbate peroxidase (−19.1 %). Taken together, the results indicate that a beneficial bacterial consortium comprising Bradyrhizobium spp. and A. brasilense strains combined with MSM application can alleviate oxidative damage during dry spells. Furthermore, this consortium improved soybean nodulation, plant growth development, and grain yield by up to 12.2 %.

Originele taal-2	Engels
Artikelnummer	126293
Tijdschrift	European Journal of Agronomy
Volume	127
Vroegere onlinedatum	2021
DOI's	https://doi.org/10.1016/j.eja.2021.126293
Status	Gepubliceerd - 2021

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10.1016/j.eja.2021.126293Licentie: CC BY

7188_MorettiFinal published version, 6,96 MBLicentie: CC BY

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Moretti de Souza, L., Crusciol, C. A. C., Bossolani, J. W., Calonego, J. C., Moreira, A., Garcia, A., Momesso, L., Kuramae, E., & Hungria, M. (2021). Beneficial microbial species and metabolites alleviate soybean oxidative damage and increase grain yield during short dry spells. European Journal of Agronomy, 127, Artikel 126293. https://doi.org/10.1016/j.eja.2021.126293

@article{1321815141f44711801546d2e00f26b7,

title = "Beneficial microbial species and metabolites alleviate soybean oxidative damage and increase grain yield during short dry spells",

abstract = "Short dry spells are an important grain yield constraint in tropical regions. Plant growth-promoting bacteria (PGPB) and their metabolites can mitigate the impact of drought stress by promoting changes in plant metabolism, physiology, and biochemistry. However, the effects of PGPB on soybean [Glycine max (L.) Merril] under drought stress in tropical regions have not been established. The experiments were carried out under tropical field conditions with short dry spells. Therefore, in this study we used a three-factorial trial to evaluate the effects of bacterial consortium consisting of N2-fixing Bradyrhizobium japonicum (strain SEMIA 5079) and Bradyrhizobium diazoefficiens (strain SEMIA 5080), the biocontrol agent Bacillus subtilis (strain QST 713), and the plant growth-promoting Azospirillum brasilense (strains Ab-V5 and Ab-V6) with or without application of microbial secondary metabolites (MSM, rhizobial metabolites enriched in lipo-chitooligosaccharides (LCOs)) during two growing seasons. Photosynthetic pigments, gas exchange parameters, antioxidant enzyme activity and proline concentrations in leaves, nodulation, plant growth development and grain yield were evaluated. The bacterial consortium comprising Bradyrhizobium spp., A. brasilense strains and MSM application increased the contents of chlorophyll a (14.5 %), chlorophyll b (30.8 %), total chlorophyll (17.2 %), and total carotenoids (27.3 %) compared with Bradyrhizobium spp. treatment alone. This consortium also increased the net photosynthetic rate (17.7 %), stomatal conductance (56.5 %), internal CO2 concentration in the substomatal chamber (8.3 %), and transpiration (44 %) compared with plants that received the standard inoculation (Bradyrhizobium spp. only), while reducing the leaf contents of hydrogen peroxide (−18.8 %) and proline (−29.4 %), lipid peroxidation (−15.9 %), and the activities of superoxide dismutase (−18.2 %), catalase (−21.2 %), and ascorbate peroxidase (−19.1 %). Taken together, the results indicate that a beneficial bacterial consortium comprising Bradyrhizobium spp. and A. brasilense strains combined with MSM application can alleviate oxidative damage during dry spells. Furthermore, this consortium improved soybean nodulation, plant growth development, and grain yield by up to 12.2 %.",

keywords = "international, Plan_S-Compliant_OA",

author = "{Moretti de Souza}, Luiz and Crusciol, {Carlos A. C.} and Bossolani, {Jo{\~a}o William} and Calonego, {Juliano C.} and Ad{\^o}nis Moreira and Ariani Garcia and L. Momesso and Eiko Kuramae and Mariangela Hungria",

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year = "2021",

doi = "10.1016/j.eja.2021.126293",

language = "English",

volume = "127",

journal = "European Journal of Agronomy",

issn = "1161-0301",

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}

Beneficial microbial species and metabolites alleviate soybean oxidative damage and increase grain yield during short dry spells. / Moretti de Souza, Luiz; Crusciol, Carlos A. C. (Co-auteur); Bossolani, João William et al.
In: European Journal of Agronomy, Vol. 127, 126293, 2021.

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

TY - JOUR

T1 - Beneficial microbial species and metabolites alleviate soybean oxidative damage and increase grain yield during short dry spells

AU - Moretti de Souza, Luiz

AU - Crusciol, Carlos A. C.

AU - Bossolani, João William

AU - Calonego, Juliano C.

AU - Moreira, Adônis

AU - Garcia, Ariani

AU - Momesso, L.

AU - Kuramae, Eiko

AU - Hungria, Mariangela

N1 - 7188, ME; Data archiving: not applicable

PY - 2021

Y1 - 2021

N2 - Short dry spells are an important grain yield constraint in tropical regions. Plant growth-promoting bacteria (PGPB) and their metabolites can mitigate the impact of drought stress by promoting changes in plant metabolism, physiology, and biochemistry. However, the effects of PGPB on soybean [Glycine max (L.) Merril] under drought stress in tropical regions have not been established. The experiments were carried out under tropical field conditions with short dry spells. Therefore, in this study we used a three-factorial trial to evaluate the effects of bacterial consortium consisting of N2-fixing Bradyrhizobium japonicum (strain SEMIA 5079) and Bradyrhizobium diazoefficiens (strain SEMIA 5080), the biocontrol agent Bacillus subtilis (strain QST 713), and the plant growth-promoting Azospirillum brasilense (strains Ab-V5 and Ab-V6) with or without application of microbial secondary metabolites (MSM, rhizobial metabolites enriched in lipo-chitooligosaccharides (LCOs)) during two growing seasons. Photosynthetic pigments, gas exchange parameters, antioxidant enzyme activity and proline concentrations in leaves, nodulation, plant growth development and grain yield were evaluated. The bacterial consortium comprising Bradyrhizobium spp., A. brasilense strains and MSM application increased the contents of chlorophyll a (14.5 %), chlorophyll b (30.8 %), total chlorophyll (17.2 %), and total carotenoids (27.3 %) compared with Bradyrhizobium spp. treatment alone. This consortium also increased the net photosynthetic rate (17.7 %), stomatal conductance (56.5 %), internal CO2 concentration in the substomatal chamber (8.3 %), and transpiration (44 %) compared with plants that received the standard inoculation (Bradyrhizobium spp. only), while reducing the leaf contents of hydrogen peroxide (−18.8 %) and proline (−29.4 %), lipid peroxidation (−15.9 %), and the activities of superoxide dismutase (−18.2 %), catalase (−21.2 %), and ascorbate peroxidase (−19.1 %). Taken together, the results indicate that a beneficial bacterial consortium comprising Bradyrhizobium spp. and A. brasilense strains combined with MSM application can alleviate oxidative damage during dry spells. Furthermore, this consortium improved soybean nodulation, plant growth development, and grain yield by up to 12.2 %.

AB - Short dry spells are an important grain yield constraint in tropical regions. Plant growth-promoting bacteria (PGPB) and their metabolites can mitigate the impact of drought stress by promoting changes in plant metabolism, physiology, and biochemistry. However, the effects of PGPB on soybean [Glycine max (L.) Merril] under drought stress in tropical regions have not been established. The experiments were carried out under tropical field conditions with short dry spells. Therefore, in this study we used a three-factorial trial to evaluate the effects of bacterial consortium consisting of N2-fixing Bradyrhizobium japonicum (strain SEMIA 5079) and Bradyrhizobium diazoefficiens (strain SEMIA 5080), the biocontrol agent Bacillus subtilis (strain QST 713), and the plant growth-promoting Azospirillum brasilense (strains Ab-V5 and Ab-V6) with or without application of microbial secondary metabolites (MSM, rhizobial metabolites enriched in lipo-chitooligosaccharides (LCOs)) during two growing seasons. Photosynthetic pigments, gas exchange parameters, antioxidant enzyme activity and proline concentrations in leaves, nodulation, plant growth development and grain yield were evaluated. The bacterial consortium comprising Bradyrhizobium spp., A. brasilense strains and MSM application increased the contents of chlorophyll a (14.5 %), chlorophyll b (30.8 %), total chlorophyll (17.2 %), and total carotenoids (27.3 %) compared with Bradyrhizobium spp. treatment alone. This consortium also increased the net photosynthetic rate (17.7 %), stomatal conductance (56.5 %), internal CO2 concentration in the substomatal chamber (8.3 %), and transpiration (44 %) compared with plants that received the standard inoculation (Bradyrhizobium spp. only), while reducing the leaf contents of hydrogen peroxide (−18.8 %) and proline (−29.4 %), lipid peroxidation (−15.9 %), and the activities of superoxide dismutase (−18.2 %), catalase (−21.2 %), and ascorbate peroxidase (−19.1 %). Taken together, the results indicate that a beneficial bacterial consortium comprising Bradyrhizobium spp. and A. brasilense strains combined with MSM application can alleviate oxidative damage during dry spells. Furthermore, this consortium improved soybean nodulation, plant growth development, and grain yield by up to 12.2 %.

KW - international

KW - Plan_S-Compliant_OA

U2 - 10.1016/j.eja.2021.126293

DO - 10.1016/j.eja.2021.126293

M3 - Article

SN - 1161-0301

VL - 127

JO - European Journal of Agronomy

JF - European Journal of Agronomy

M1 - 126293

ER -

Beneficial microbial species and metabolites alleviate soybean oxidative damage and increase grain yield during short dry spells

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