Biolistic transformation of Caenorhabditis elegans

TY - JOUR

T1 - Biolistic transformation of Caenorhabditis elegans

AU - Isik, M.

AU - Berezikov, E.

N1 - Reporting year: 2013

PY - 2013

Y1 - 2013

N2 - The ability to generate transgenic animals to study gene expression and function is a powerful and important part of the Caenorhabditis elegans genetic toolbox. Transgenic animals can be created by introducing exogenous DNA into the worm germline either by microinjection or by microparticle bombardment (biolistic transformation). In this chapter we describe a simple and robust protocol to generate transgenic C. elegans animals by biolistic transformation with gold particles using the Bio-Rad PDS-1000/He system with Hepta adapter and unc-119 selection marker. We also point out the steps that need special attention to achieve successful transformations.

AB - The ability to generate transgenic animals to study gene expression and function is a powerful and important part of the Caenorhabditis elegans genetic toolbox. Transgenic animals can be created by introducing exogenous DNA into the worm germline either by microinjection or by microparticle bombardment (biolistic transformation). In this chapter we describe a simple and robust protocol to generate transgenic C. elegans animals by biolistic transformation with gold particles using the Bio-Rad PDS-1000/He system with Hepta adapter and unc-119 selection marker. We also point out the steps that need special attention to achieve successful transformations.

U2 - 10.1007/978-1-62703-110-3_7

DO - 10.1007/978-1-62703-110-3_7

M3 - Article

SN - 1064-3745

VL - 940

SP - 77

EP - 86

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -