Chaperoning of the histone octamer by the acidic domain of DNA repair factor APLF

Ivan Corbeski; Xiaohu Guo; Bruna V Eckhardt; Domenico Fasci; Wouter Wiegant; Melissa A Graewert; Kees Vreeken; Hans Wienk; Dmitri I Svergun; Albert J R Heck; Haico van Attikum; Rolf Boelens; Titia K Sixma; Francesca Mattiroli; Hugo van Ingen

doi:10.1126/sciadv.abo0517

Chaperoning of the histone octamer by the acidic domain of DNA repair factor APLF

Ivan Corbeski, Xiaohu Guo, Bruna V Eckhardt, Domenico Fasci, Wouter Wiegant, Melissa A Graewert, Kees Vreeken, Hans Wienk, Dmitri I Svergun, Albert J R Heck, Haico van Attikum, Rolf Boelens, Titia K Sixma, Francesca Mattiroli, Hugo van Ingen

Hubrecht Institute

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

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Nucleosome assembly requires the coordinated deposition of histone complexes H3-H4 and H2A-H2B to form a histone octamer on DNA. In the current paradigm, specific histone chaperones guide the deposition of first H3-H4 and then H2A-H2B. Here, we show that the acidic domain of DNA repair factor APLF (APLFAD) can assemble the histone octamer in a single step and deposit it on DNA to form nucleosomes. The crystal structure of the APLFAD-histone octamer complex shows that APLFAD tethers the histones in their nucleosomal conformation. Mutations of key aromatic anchor residues in APLFAD affect chaperone activity in vitro and in cells. Together, we propose that chaperoning of the histone octamer is a mechanism for histone chaperone function at sites where chromatin is temporarily disrupted.

Originele taal-2	Engels
Pagina's (van-tot)	eabo0517
Tijdschrift	Science advances
Volume	8
Nummer van het tijdschrift	30
DOI's	https://doi.org/10.1126/sciadv.abo0517
Status	Gepubliceerd - 29 jul. 2022

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Corbeski, I., Guo, X., Eckhardt, B. V., Fasci, D., Wiegant, W., Graewert, M. A., Vreeken, K., Wienk, H., Svergun, D. I., Heck, A. J. R., van Attikum, H., Boelens, R., Sixma, T. K., Mattiroli, F., & van Ingen, H. (2022). Chaperoning of the histone octamer by the acidic domain of DNA repair factor APLF. Science advances, 8(30), eabo0517. https://doi.org/10.1126/sciadv.abo0517

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title = "Chaperoning of the histone octamer by the acidic domain of DNA repair factor APLF",

abstract = "Nucleosome assembly requires the coordinated deposition of histone complexes H3-H4 and H2A-H2B to form a histone octamer on DNA. In the current paradigm, specific histone chaperones guide the deposition of first H3-H4 and then H2A-H2B. Here, we show that the acidic domain of DNA repair factor APLF (APLFAD) can assemble the histone octamer in a single step and deposit it on DNA to form nucleosomes. The crystal structure of the APLFAD-histone octamer complex shows that APLFAD tethers the histones in their nucleosomal conformation. Mutations of key aromatic anchor residues in APLFAD affect chaperone activity in vitro and in cells. Together, we propose that chaperoning of the histone octamer is a mechanism for histone chaperone function at sites where chromatin is temporarily disrupted.",

keywords = "DNA/chemistry, DNA Repair, Histone Chaperones/genetics, Histones/metabolism, Molecular Chaperones/genetics, Nucleosomes",

author = "Ivan Corbeski and Xiaohu Guo and Eckhardt, {Bruna V} and Domenico Fasci and Wouter Wiegant and Graewert, {Melissa A} and Kees Vreeken and Hans Wienk and Svergun, {Dmitri I} and Heck, {Albert J R} and {van Attikum}, Haico and Rolf Boelens and Sixma, {Titia K} and Francesca Mattiroli and {van Ingen}, Hugo",

year = "2022",

month = jul,

day = "29",

doi = "10.1126/sciadv.abo0517",

language = "English",

volume = "8",

pages = "eabo0517",

journal = "Science advances",

issn = "2375-2548",

publisher = "American Association for the Advancement of Science (AAAS)",

number = "30",

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TY - JOUR

T1 - Chaperoning of the histone octamer by the acidic domain of DNA repair factor APLF

AU - Corbeski, Ivan

AU - Guo, Xiaohu

AU - Eckhardt, Bruna V

AU - Fasci, Domenico

AU - Wiegant, Wouter

AU - Graewert, Melissa A

AU - Vreeken, Kees

AU - Wienk, Hans

AU - Svergun, Dmitri I

AU - Heck, Albert J R

AU - van Attikum, Haico

AU - Boelens, Rolf

AU - Sixma, Titia K

AU - Mattiroli, Francesca

AU - van Ingen, Hugo

PY - 2022/7/29

Y1 - 2022/7/29

N2 - Nucleosome assembly requires the coordinated deposition of histone complexes H3-H4 and H2A-H2B to form a histone octamer on DNA. In the current paradigm, specific histone chaperones guide the deposition of first H3-H4 and then H2A-H2B. Here, we show that the acidic domain of DNA repair factor APLF (APLFAD) can assemble the histone octamer in a single step and deposit it on DNA to form nucleosomes. The crystal structure of the APLFAD-histone octamer complex shows that APLFAD tethers the histones in their nucleosomal conformation. Mutations of key aromatic anchor residues in APLFAD affect chaperone activity in vitro and in cells. Together, we propose that chaperoning of the histone octamer is a mechanism for histone chaperone function at sites where chromatin is temporarily disrupted.

AB - Nucleosome assembly requires the coordinated deposition of histone complexes H3-H4 and H2A-H2B to form a histone octamer on DNA. In the current paradigm, specific histone chaperones guide the deposition of first H3-H4 and then H2A-H2B. Here, we show that the acidic domain of DNA repair factor APLF (APLFAD) can assemble the histone octamer in a single step and deposit it on DNA to form nucleosomes. The crystal structure of the APLFAD-histone octamer complex shows that APLFAD tethers the histones in their nucleosomal conformation. Mutations of key aromatic anchor residues in APLFAD affect chaperone activity in vitro and in cells. Together, we propose that chaperoning of the histone octamer is a mechanism for histone chaperone function at sites where chromatin is temporarily disrupted.

KW - DNA/chemistry

KW - DNA Repair

KW - Histone Chaperones/genetics

KW - Histones/metabolism

KW - Molecular Chaperones/genetics

KW - Nucleosomes

U2 - 10.1126/sciadv.abo0517

DO - 10.1126/sciadv.abo0517

M3 - Article

C2 - 35895815

SN - 2375-2548

VL - 8

SP - eabo0517

JO - Science advances

JF - Science advances

IS - 30

ER -

Chaperoning of the histone octamer by the acidic domain of DNA repair factor APLF

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