Characterization of novel Acidobacteria exopolysaccharides with potential industrial and ecological applications

TY - JOUR

T1 - Characterization of novel Acidobacteria exopolysaccharides with potential industrial and ecological applications

AU - Kielak, A.M.

AU - Castellane, T.C.L.

AU - Campanharo, J.C.

AU - Colnago, L.A.

AU - Costa, Ohana Y.A.

AU - Corradi da Silva, M.L.

AU - van Veen, J.A.

AU - Lemos, E.G.

AU - Kuramae, E.E.

N1 - 6219, ME; Data archiving: data archived at publisher

PY - 2017

Y1 - 2017

N2 - Acidobacteria have been described as one of the most abundant and ubiquitous bacterial phyla in soil.However, factors contributing to this ecological success are not well elucidated mainly due to difficultiesin bacterial isolation. Acidobacteria may be able to survive for long periods in soil due to protectionprovided by secreted extracellular polymeric substances that include exopolysaccharides (EPSs).Here we present the first study to characterize EPSs derived from two strains of Acidobacteria fromsubdivision 1 belonging to Granulicella sp. EPS are unique heteropolysaccharides containing mannose,glucose, galactose and xylose as major components, and are modified with carboxyl and methoxylfunctional groups that we characterized by Fourier transform infrared (FTIR) spectroscopy. BothEPS compounds we identified can efficiently emulsify various oils (sunflower seed, diesel, and liquidparaffin) and hydrocarbons (toluene and hexane). Moreover, the emulsions are more thermostable overtime than those of commercialized xanthan. Acidobacterial EPS can now be explored as a source ofbiopolymers that may be attractive and valuable for industrial applications due to their natural origin,sustainability, biodegradability and low toxicity.

AB - Acidobacteria have been described as one of the most abundant and ubiquitous bacterial phyla in soil.However, factors contributing to this ecological success are not well elucidated mainly due to difficultiesin bacterial isolation. Acidobacteria may be able to survive for long periods in soil due to protectionprovided by secreted extracellular polymeric substances that include exopolysaccharides (EPSs).Here we present the first study to characterize EPSs derived from two strains of Acidobacteria fromsubdivision 1 belonging to Granulicella sp. EPS are unique heteropolysaccharides containing mannose,glucose, galactose and xylose as major components, and are modified with carboxyl and methoxylfunctional groups that we characterized by Fourier transform infrared (FTIR) spectroscopy. BothEPS compounds we identified can efficiently emulsify various oils (sunflower seed, diesel, and liquidparaffin) and hydrocarbons (toluene and hexane). Moreover, the emulsions are more thermostable overtime than those of commercialized xanthan. Acidobacterial EPS can now be explored as a source ofbiopolymers that may be attractive and valuable for industrial applications due to their natural origin,sustainability, biodegradability and low toxicity.

KW - international

U2 - 10.1038/srep41193

DO - 10.1038/srep41193

M3 - Article

SN - 2045-2322

VL - 7

JO - Scientific Reports

JF - Scientific Reports

M1 - 41193

ER -