Clonal population of flucytosine-resistant Candida tropicalis from blood cultures, Paris, France

TY - JOUR

T1 - Clonal population of flucytosine-resistant Candida tropicalis from blood cultures, Paris, France

AU - Desnos-Ollivier, Marie

AU - Bretagne, Stéphane

AU - Bernède, Claire

AU - Robert, Vincent

AU - Raoux, Dorothée

AU - Chachaty, Elisabeth

AU - Forget, Elisabeth

AU - Lacroix, Claire

AU - Dromer, Françoise

PY - 2008/4

Y1 - 2008/4

N2 - Candida tropicalis is a diploid ascomycetes yeast responsible for 4%-24% of candidemia. Resistance to flucytosine is rarely described for this species but was observed for 45 (35%) of 130 C. tropicalis isolates recovered from blood cultures in the Paris area in a 4-year survey. The aims of this study were to test the hypothesis that the flucytosine-resistant isolates could represent a subgroup and to determine the relationship between epidemiologic and genomic data. Epidemiologic data and gene sequences were analyzed, and molecular typing was performed. Our results suggest that a clone of flucytosine-resistant isolates, associated with malignancies and a lower mortality than that for other C. tropicalis isolates, is widespread in the Paris area. We propose the analysis of 2 polymorphic microsatellite markers coupled with URA3 sequencing to track the clone.

AB - Candida tropicalis is a diploid ascomycetes yeast responsible for 4%-24% of candidemia. Resistance to flucytosine is rarely described for this species but was observed for 45 (35%) of 130 C. tropicalis isolates recovered from blood cultures in the Paris area in a 4-year survey. The aims of this study were to test the hypothesis that the flucytosine-resistant isolates could represent a subgroup and to determine the relationship between epidemiologic and genomic data. Epidemiologic data and gene sequences were analyzed, and molecular typing was performed. Our results suggest that a clone of flucytosine-resistant isolates, associated with malignancies and a lower mortality than that for other C. tropicalis isolates, is widespread in the Paris area. We propose the analysis of 2 polymorphic microsatellite markers coupled with URA3 sequencing to track the clone.

KW - Antifungal Agents

KW - Candida tropicalis

KW - Candidiasis

KW - Drug Resistance, Fungal

KW - Flucytosine

KW - France

KW - Fungemia

KW - Genotype

KW - Humans

KW - Microsatellite Repeats

KW - Phenotype

KW - Risk Factors

KW - Time Factors

U2 - 10.3201/eid1404.071083

DO - 10.3201/eid1404.071083

M3 - Article

C2 - 18394272

SN - 1080-6040

VL - 14

SP - 557

EP - 565

JO - Emerging Infectious Diseases

JF - Emerging Infectious Diseases

IS - 4

ER -