Combination of amphotericin B and flucytosine against neurotropic species of melanized fungi causing Primary cerebral phaeohyphomycosis

S. Deng; W. Pan; W. Liao; G. S. De Hoog; A. H G Gerrits Van Den Ende; R. G. Vitale; H. Rafati; M. Ilkit; A. H. Van Der Lee; A. J M M Rijs; P. E. Verweij; S. Seyedmousavi

doi:10.1128/AAC.02526-15

Combination of amphotericin B and flucytosine against neurotropic species of melanized fungi causing Primary cerebral phaeohyphomycosis

S. Deng, W. Pan, W. Liao, G. S. De Hoog, A. H G Gerrits Van Den Ende, R. G. Vitale, H. Rafati, M. Ilkit, A. H. Van Der Lee, A. J M M Rijs, P. E. Verweij, S. Seyedmousavi

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

22 Citaten (Scopus)

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Primary central nervous system phaeohyphomycosis is a fatal fungal infection due mainly to the neurotropic melanized fungi Cladophialophora bantiana , Rhinocladiella mackenziei , and Exophiala dermatitidis. Despite the combination of surgery with antifungal treatment, the prognosis continues to be poor, with mortality rates ranging from 50 to 70%. Therefore, a search for a more-appropriate therapeutic approach is urgently needed. Our in vitro studies showed that with the combination of amphotericin B and flucytosine against these species, the median fractional inhibitory concentration (FIC) indices for strains ranged from 0.25 to 0.38, indicating synergy. By use of Bliss independence analysis, a significant degree of synergy was confirmed for all strains, with the sum ΔE ranging from 90.2 to 698.61%. No antagonism was observed. These results indicate that amphotericin B, in combination with flucytosine, may have a role in the treatment of primary cerebral infections caused by melanized fungi belonging to the order Chaetothyriales . Further in vivo studies and clinical investigations to elucidate and confirm these observations are warranted.

Originele taal-2	Engels
Pagina's (van-tot)	2346-2351
Aantal pagina's	6
Tijdschrift	Antimicrobial Agents and Chemotherapy
Volume	60
Nummer van het tijdschrift	4
DOI's	https://doi.org/10.1128/AAC.02526-15
Status	Gepubliceerd - 01 apr. 2016

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Deng, S., Pan, W., Liao, W., De Hoog, G. S., Van Den Ende, A. H. G. G., Vitale, R. G., Rafati, H., Ilkit, M., Van Der Lee, A. H., Rijs, A. J. M. M., Verweij, P. E., & Seyedmousavi, S. (2016). Combination of amphotericin B and flucytosine against neurotropic species of melanized fungi causing Primary cerebral phaeohyphomycosis. Antimicrobial Agents and Chemotherapy, 60(4), 2346-2351. https://doi.org/10.1128/AAC.02526-15

@article{7a5777c7a37f44a8b695e6e4fb606094,

title = "Combination of amphotericin B and flucytosine against neurotropic species of melanized fungi causing Primary cerebral phaeohyphomycosis",

abstract = " Primary central nervous system phaeohyphomycosis is a fatal fungal infection due mainly to the neurotropic melanized fungi Cladophialophora bantiana , Rhinocladiella mackenziei , and Exophiala dermatitidis. Despite the combination of surgery with antifungal treatment, the prognosis continues to be poor, with mortality rates ranging from 50 to 70%. Therefore, a search for a more-appropriate therapeutic approach is urgently needed. Our in vitro studies showed that with the combination of amphotericin B and flucytosine against these species, the median fractional inhibitory concentration (FIC) indices for strains ranged from 0.25 to 0.38, indicating synergy. By use of Bliss independence analysis, a significant degree of synergy was confirmed for all strains, with the sum ΔE ranging from 90.2 to 698.61%. No antagonism was observed. These results indicate that amphotericin B, in combination with flucytosine, may have a role in the treatment of primary cerebral infections caused by melanized fungi belonging to the order Chaetothyriales . Further in vivo studies and clinical investigations to elucidate and confirm these observations are warranted. ",

author = "S. Deng and W. Pan and W. Liao and {De Hoog}, {G. S.} and {Van Den Ende}, {A. H G Gerrits} and Vitale, {R. G.} and H. Rafati and M. Ilkit and {Van Der Lee}, {A. H.} and Rijs, {A. J M M} and Verweij, {P. E.} and S. Seyedmousavi",

year = "2016",

month = apr,

day = "1",

doi = "10.1128/AAC.02526-15",

language = "English",

volume = "60",

pages = "2346--2351",

journal = "Antimicrobial Agents and Chemotherapy",

issn = "0066-4804",

publisher = "American Society for Microbiology",

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Deng, S, Pan, W, Liao, W, De Hoog, GS, Van Den Ende, AHGG, Vitale, RG, Rafati, H, Ilkit, M, Van Der Lee, AH, Rijs, AJMM, Verweij, PE & Seyedmousavi, S 2016, 'Combination of amphotericin B and flucytosine against neurotropic species of melanized fungi causing Primary cerebral phaeohyphomycosis', Antimicrobial Agents and Chemotherapy, vol. 60, nr. 4, blz. 2346-2351. https://doi.org/10.1128/AAC.02526-15

Combination of amphotericin B and flucytosine against neurotropic species of melanized fungi causing Primary cerebral phaeohyphomycosis. / Deng, S.; Pan, W.; Liao, W. et al.
In: Antimicrobial Agents and Chemotherapy, Vol. 60, Nr. 4, 01.04.2016, blz. 2346-2351.

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

TY - JOUR

T1 - Combination of amphotericin B and flucytosine against neurotropic species of melanized fungi causing Primary cerebral phaeohyphomycosis

AU - Deng, S.

AU - Pan, W.

AU - Liao, W.

AU - De Hoog, G. S.

AU - Van Den Ende, A. H G Gerrits

AU - Vitale, R. G.

AU - Rafati, H.

AU - Ilkit, M.

AU - Van Der Lee, A. H.

AU - Rijs, A. J M M

AU - Verweij, P. E.

AU - Seyedmousavi, S.

PY - 2016/4/1

Y1 - 2016/4/1

N2 - Primary central nervous system phaeohyphomycosis is a fatal fungal infection due mainly to the neurotropic melanized fungi Cladophialophora bantiana , Rhinocladiella mackenziei , and Exophiala dermatitidis. Despite the combination of surgery with antifungal treatment, the prognosis continues to be poor, with mortality rates ranging from 50 to 70%. Therefore, a search for a more-appropriate therapeutic approach is urgently needed. Our in vitro studies showed that with the combination of amphotericin B and flucytosine against these species, the median fractional inhibitory concentration (FIC) indices for strains ranged from 0.25 to 0.38, indicating synergy. By use of Bliss independence analysis, a significant degree of synergy was confirmed for all strains, with the sum ΔE ranging from 90.2 to 698.61%. No antagonism was observed. These results indicate that amphotericin B, in combination with flucytosine, may have a role in the treatment of primary cerebral infections caused by melanized fungi belonging to the order Chaetothyriales . Further in vivo studies and clinical investigations to elucidate and confirm these observations are warranted.

AB - Primary central nervous system phaeohyphomycosis is a fatal fungal infection due mainly to the neurotropic melanized fungi Cladophialophora bantiana , Rhinocladiella mackenziei , and Exophiala dermatitidis. Despite the combination of surgery with antifungal treatment, the prognosis continues to be poor, with mortality rates ranging from 50 to 70%. Therefore, a search for a more-appropriate therapeutic approach is urgently needed. Our in vitro studies showed that with the combination of amphotericin B and flucytosine against these species, the median fractional inhibitory concentration (FIC) indices for strains ranged from 0.25 to 0.38, indicating synergy. By use of Bliss independence analysis, a significant degree of synergy was confirmed for all strains, with the sum ΔE ranging from 90.2 to 698.61%. No antagonism was observed. These results indicate that amphotericin B, in combination with flucytosine, may have a role in the treatment of primary cerebral infections caused by melanized fungi belonging to the order Chaetothyriales . Further in vivo studies and clinical investigations to elucidate and confirm these observations are warranted.

U2 - 10.1128/AAC.02526-15

DO - 10.1128/AAC.02526-15

M3 - Article

C2 - 26833164

SN - 0066-4804

VL - 60

SP - 2346

EP - 2351

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

IS - 4

ER -

Combination of amphotericin B and flucytosine against neurotropic species of melanized fungi causing Primary cerebral phaeohyphomycosis

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