Competition for nitrate and glucose between Pseudomonas fluorescens and Bacillus licheniformis under continuous or fluctuating anoxic conditions

TY - JOUR

T1 - Competition for nitrate and glucose between Pseudomonas fluorescens and Bacillus licheniformis under continuous or fluctuating anoxic conditions

AU - Nijburg, J.W.

AU - Gerards, S.

AU - Laanbroek, H.J.

N1 - Reporting year: 1998 Metis note: 2355; CTE ; CL; TME ; MWE ; ME file:///L:/Endnotedatabases/NIOOPUB/pdfs/Pdfs1998/Nijburg_ea_2355.pdf

PY - 1998

Y1 - 1998

N2 - The dissimilatory nitrate-reducing bacterial community in the rhizosphere of aerenchymatous plant species such as Glyceria maxima, consists of oxidative. denitrifying and fermentative nitrate-ammonifying bacteria. To study the respective ecological niches of both types of nitrate-reducing bacteria, competition for nitrate or glucose between the representative denitrifier Pseudomonas fluorescens and the representative fermentative nitrate-ammonifying Bacillus licheniformis under continuous or fluctuating anoxic conditions were performed in continuous culture. Competition started by mixing the separate, steady-slate mono-cultures of the two species at different ratios. All the experiments were performed at a dilution rate of 0.05 h(-1). The competition was followed by measuring concentrations of nitrogen, glucose and fatty acids and by determining the cell numbers of P. fluorescens and B. licheniformis. Under continuous anoxic nitrate-limited conditions and under certain fluctuating anoxic conditions (8 h 10% and 16 h 0% air saturation), B. licheniformis was able to maintain itself in the chemostat at a low percentage of 4-7%. Under continuous anoxic glucose-limited conditions and under specific fluctuating anoxic (16 h 10% and 8 h 0% air saturation) conditions, B. licheniformis washed out. The outcome of the competition was explained by a higher affinity of P. fluorescens for nitrate arid glucose compared to B. licheniformis. B. licheniformis was able to maintain itself in the chemostat under continuous anoxic nitrate-limited conditions and under certain fluctuating anoxic conditions (8 h 10% and 16 h 0% air saturation) due to the fer;mentation of the remaining glucose. [KEYWORDS: competition; nitrate reduction; chemostat; Pseudomonas fluorescens; Bacillus licheniformis Denitrification; oxygen; rhizosphere; transient; culture]

AB - The dissimilatory nitrate-reducing bacterial community in the rhizosphere of aerenchymatous plant species such as Glyceria maxima, consists of oxidative. denitrifying and fermentative nitrate-ammonifying bacteria. To study the respective ecological niches of both types of nitrate-reducing bacteria, competition for nitrate or glucose between the representative denitrifier Pseudomonas fluorescens and the representative fermentative nitrate-ammonifying Bacillus licheniformis under continuous or fluctuating anoxic conditions were performed in continuous culture. Competition started by mixing the separate, steady-slate mono-cultures of the two species at different ratios. All the experiments were performed at a dilution rate of 0.05 h(-1). The competition was followed by measuring concentrations of nitrogen, glucose and fatty acids and by determining the cell numbers of P. fluorescens and B. licheniformis. Under continuous anoxic nitrate-limited conditions and under certain fluctuating anoxic conditions (8 h 10% and 16 h 0% air saturation), B. licheniformis was able to maintain itself in the chemostat at a low percentage of 4-7%. Under continuous anoxic glucose-limited conditions and under specific fluctuating anoxic (16 h 10% and 8 h 0% air saturation) conditions, B. licheniformis washed out. The outcome of the competition was explained by a higher affinity of P. fluorescens for nitrate arid glucose compared to B. licheniformis. B. licheniformis was able to maintain itself in the chemostat under continuous anoxic nitrate-limited conditions and under certain fluctuating anoxic conditions (8 h 10% and 16 h 0% air saturation) due to the fer;mentation of the remaining glucose. [KEYWORDS: competition; nitrate reduction; chemostat; Pseudomonas fluorescens; Bacillus licheniformis Denitrification; oxygen; rhizosphere; transient; culture]

U2 - 10.1111/j.1574-6941.1998.tb00519.x

DO - 10.1111/j.1574-6941.1998.tb00519.x

M3 - Article

SN - 0168-6496

VL - 26

SP - 345

EP - 356

JO - FEMS Microbiology Ecology

JF - FEMS Microbiology Ecology

IS - 4

ER -