Constitutional chromothripsis rearrangements involve clustered double-stranded DNA breaks and nonhomologous repair mechanisms

W.P. Kloosterman, M. Tavakoli-Yaraki, M.J. van Roosmalen, E. van Binsbergen, I. Renkens, K. Duran, L. Ballarati, S. Vergult, D. Giardino, K. Hansson, C.A. Ruivenkamp, M. de Jager, A. van Haeringen, E.F. Ippel, T. Haaf, E. Passarge, R. Hochstenbach, B. Menten, L. Larizza, V. GuryevM. Poot, E. Cuppen

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgaveArtikelWetenschappelijkpeer review

Samenvatting

Chromothripsis represents a novel phenomenon in the structural variation landscape of cancer genomes. Here, we analyze the genomes of ten patients with congenital disease who were preselected to carry complex chromosomal rearrangements with more than two breakpoints. The rearrangements displayed unanticipated complexity resembling chromothripsis. We find that eight of them contain hallmarks of multiple clustered double-stranded DNA breaks (DSBs) on one or more chromosomes. In addition, nucleotide resolution analysis of 98 breakpoint junctions indicates that break repair involves nonhomologous or microhomology-mediated end joining. We observed that these eight rearrangements are balanced or contain sporadic deletions ranging in size between a few hundred base pairs and several megabases. The two remaining complex rearrangements did not display signs of DSBs and contain duplications, indicative of rearrangement processes involving template switching. Our work provides detailed insight into the characteristics of chromothripsis and supports a role for clustered DSBs driving some constitutional chromothripsis rearrangements.
Originele taal-2Engels
Pagina's (van-tot)648-655
TijdschriftCell Reports
Volume1
Nummer van het tijdschrift6
DOI's
StatusGepubliceerd - 2012

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