Controlled Release of RNAi Molecules by Tunable Supramolecular Hydrogel Carriers

Maarten H Bakker; Eva van Rooij; Patricia Y W Dankers

doi:10.1002/asia.201800582

Controlled Release of RNAi Molecules by Tunable Supramolecular Hydrogel Carriers

Maarten H Bakker, Eva van Rooij, Patricia Y W Dankers

Hubrecht Institute

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

21 Citaten (Scopus)

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Local, sustained release and presentation of RNAi therapeutics can be achieved with hydrogel delivery systems. Here we show the development of a supramolecular hydrogel into a local RNAi delivery system. By careful material design, two simple but effective strategies are introduced to obtain controlled release of two classes of RNAi therapeutics, that is, microRNA and antimiR. It was shown that the release of microRNA could be regulated using cholesterol-modification for interaction with the supramolecular hydrogel. Non-modified antimiR release could be controlled via supramolecular introduction of positively charged additive molecules into the supramolecular hydrogel. In this way, either the cholesterol-modification on the drug or the charge introduction into the hydrogel provides handles for controlled RNAi therapy.

Originele taal-2	Engels
Pagina's (van-tot)	3501-3508
Aantal pagina's	8
Tijdschrift	Chemistry - An Asian Journal
Volume	13
Nummer van het tijdschrift	22
DOI's	https://doi.org/10.1002/asia.201800582
Status	Gepubliceerd - 16 nov. 2018

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10.1002/asia.201800582

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title = "Controlled Release of RNAi Molecules by Tunable Supramolecular Hydrogel Carriers",

abstract = "Local, sustained release and presentation of RNAi therapeutics can be achieved with hydrogel delivery systems. Here we show the development of a supramolecular hydrogel into a local RNAi delivery system. By careful material design, two simple but effective strategies are introduced to obtain controlled release of two classes of RNAi therapeutics, that is, microRNA and antimiR. It was shown that the release of microRNA could be regulated using cholesterol-modification for interaction with the supramolecular hydrogel. Non-modified antimiR release could be controlled via supramolecular introduction of positively charged additive molecules into the supramolecular hydrogel. In this way, either the cholesterol-modification on the drug or the charge introduction into the hydrogel provides handles for controlled RNAi therapy.",

keywords = "Antagomirs/chemistry, Cholesterol/chemistry, Drug Carriers/chemistry, Drug Liberation, Fluorescence Recovery After Photobleaching, HEK293 Cells, Humans, Hydrogels/chemistry, Hydrogen-Ion Concentration, Polyethylene Glycols/chemistry, RNA Interference, RNA, Small Interfering/chemistry, Rheology, Viscosity",

author = "Bakker, {Maarten H} and {van Rooij}, Eva and Dankers, {Patricia Y W}",

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KW - Humans

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KW - Hydrogen-Ion Concentration

KW - Polyethylene Glycols/chemistry

KW - RNA Interference

KW - RNA, Small Interfering/chemistry

KW - Rheology

KW - Viscosity

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Controlled Release of RNAi Molecules by Tunable Supramolecular Hydrogel Carriers

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