CRISPR-Cas Tools and Their Application in Genetic Engineering of Human Stem Cells and Organoids

Delilah Hendriks, Hans Clevers, Benedetta Artegiani

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgaveBoek/film/artikelrecensieWetenschappelijk

Samenvatting

CRISPR-Cas technology has revolutionized biological research and holds great therapeutic potential. Here, we review CRISPR-Cas systems and their latest developments with an emphasis on application to human cells. We also discuss how different CRISPR-based strategies can be used to accomplish a particular genome engineering goal. We then review how different CRISPR tools have been used in genome engineering of human stem cells in vitro, covering both the pluripotent (iPSC/ESC) and somatic adult stem cell fields and, in particular, 3D organoid cultures. Finally, we discuss the progress and challenges associated with CRISPR-based genome editing of human stem cells for therapeutic use.

Originele taal-2Engels
Pagina's (van-tot)705-731
Aantal pagina's27
TijdschriftCell Stem Cell
Volume27
Nummer van het tijdschrift5
DOI's
StatusGepubliceerd - 05 nov. 2020

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