CRISPR Craze to Transform Cardiac Biology

TY - JOUR

T1 - CRISPR Craze to Transform Cardiac Biology

AU - van Kampen, Sebastiaan Johannes

AU - van Rooij, Eva

N1 - Copyright © 2019 The Author(s). Published by Elsevier Ltd.. All rights reserved.

PY - 2019/9

Y1 - 2019/9

N2 - Clustered regularly interspaced short palindromic repeats (CRISPR) has revolutionized many research areas and has rapidly become the gold standard in genome editing by outrivaling all other available tools. Its unprecedented versatility creates the opportunity to modify any aspect of gene regulation. Even though the cardiac field is starting to appreciate the potential of CRISPR, many applications to study cardiac biology and disease so far have remained untouched. In particular, CRISPR-based strategies that act independent of the homology-directed repair pathway could help circumvent issues of modifying the genome of postmitotic cardiomyocytes, which is currently limiting its utility in the heart. Here, we review current applications and future potential for the use of CRISPR to study cardiac biology and disease.

AB - Clustered regularly interspaced short palindromic repeats (CRISPR) has revolutionized many research areas and has rapidly become the gold standard in genome editing by outrivaling all other available tools. Its unprecedented versatility creates the opportunity to modify any aspect of gene regulation. Even though the cardiac field is starting to appreciate the potential of CRISPR, many applications to study cardiac biology and disease so far have remained untouched. In particular, CRISPR-based strategies that act independent of the homology-directed repair pathway could help circumvent issues of modifying the genome of postmitotic cardiomyocytes, which is currently limiting its utility in the heart. Here, we review current applications and future potential for the use of CRISPR to study cardiac biology and disease.

U2 - 10.1016/j.molmed.2019.06.008

DO - 10.1016/j.molmed.2019.06.008

M3 - Article

C2 - 31326354

SN - 1471-4914

VL - 25

SP - 791

EP - 802

JO - Trends in Molecular Medicine

JF - Trends in Molecular Medicine

IS - 9

ER -