d-Asb11 is an essential mediator of canonical Delta-Notch signalling.

S.H. Diks, M.A. Sartori da Silva, J.-L. Hillebrands, R.J. Bink, H.H. Versteeg, C. van Rooijen, A.M. Brouwers, A.B. Chitnis, M.P. Peppelenbosch, D. Zivkovic

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgaveArtikelWetenschappelijkpeer review


In canonical Delta-Notch signalling, expression of Delta activates Notch in neighbouring cells, leading to downregulation of Delta in these cells. This process of lateral inhibition results in selection of either Delta-signalling cells or Notch-signalling cells. Here we show that d-Asb11 is an important mediator of this lateral inhibition. In zebrafish embryos, morpholino oligonucleotide (MO)-mediated knockdown of d-Asb11 caused repression of specific Delta-Notch elements and their transcriptional targets, whereas these were induced when d-Asb11 was misexpressed. d-Asb11 also activated legitimate Notch reporters cell-non-autonomously in vitro and in vivo when co-expressed with a Notch reporter. However, it repressed Notch reporters when expressed in Delta-expressing cells. Consistent with these results, d-Asb11 was able to specifically ubiquitylate and degrade DeltaA both in vitro and in vivo. We conclude that d-Asb11 is a component in the regulation of Delta-Notch signalling, important in fine-tuning the lateral inhibition gradients between DeltaA and Notch through a cell non-autonomous mechanism.
Originele taal-2Engels
Pagina's (van-tot)1190-1198
TijdschriftNature Cell Biology
Nummer van het tijdschrift10
StatusGepubliceerd - 2008


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