Deciphering the genome and secondary metabolome of the plant pathogen Fusarium culmorum

TY - JOUR

T1 - Deciphering the genome and secondary metabolome of the plant pathogen Fusarium culmorum

AU - Schmidt, R.L.

AU - Durling, M.

AU - de Jager, V.C.L.

AU - Menezes, R. C.

AU - Nordkvist, E.

AU - Svatos, A.

AU - Dubey, Mohit

AU - Lauterbach, L.

AU - Dikschat, J.S.

AU - Karlsson, M.

AU - Garbeva, P.V.

N1 - 6508, ME;

PY - 2018

Y1 - 2018

N2 - Fusarium culmorum is one of the most important fungal plant pathogens that causes diseases on a wide diversity of cereal and non-cereal crops. We report herein for the first time the genome sequence of F. culmorum strain PV and its associated secondary metabolome that plays a role in the interaction with other microorganisms and contributes to its pathogenicity on plants. The genome revealed the presence of two terpene synthases, trichodiene and longiborneol synthase, which generate an array of volatile terpenes. Furthermore, we identified two gene clusters, deoxynivalenol and zearalenone, which encode for the production of mycotoxins. Linking the production of mycotoxins with in vitro bioassays, we found high virulence of F. culmorum PV on maize, barley and wheat. By using ultra-performance liquid chromatography–mass spectrometry, we confirmed several compounds important for the behaviour and lifestyle of F. culmorum. This research sets the basis for future studies in microbe–plant interactions.

AB - Fusarium culmorum is one of the most important fungal plant pathogens that causes diseases on a wide diversity of cereal and non-cereal crops. We report herein for the first time the genome sequence of F. culmorum strain PV and its associated secondary metabolome that plays a role in the interaction with other microorganisms and contributes to its pathogenicity on plants. The genome revealed the presence of two terpene synthases, trichodiene and longiborneol synthase, which generate an array of volatile terpenes. Furthermore, we identified two gene clusters, deoxynivalenol and zearalenone, which encode for the production of mycotoxins. Linking the production of mycotoxins with in vitro bioassays, we found high virulence of F. culmorum PV on maize, barley and wheat. By using ultra-performance liquid chromatography–mass spectrometry, we confirmed several compounds important for the behaviour and lifestyle of F. culmorum. This research sets the basis for future studies in microbe–plant interactions.

KW - international

U2 - 10.1093/femsec/fiy078

DO - 10.1093/femsec/fiy078

M3 - Article

SN - 0168-6496

VL - 94

JO - FEMS Microbiology Ecology

JF - FEMS Microbiology Ecology

IS - 6

M1 - fiy078

ER -