Defective zoospore encystment and suppressed cyst germination of Phytophthora palmivora caused by transient leaching treatments

J Dijksterhuis, J W Deacon

    Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgaveArtikelWetenschappelijkpeer review

    Samenvatting

    The behaviour of encysting zoospores of Phytophthora palmivora during leaching conditions was studied. Zoospores encysted and germinated successfully on polycarbonate membranes after mechanical agitation. Transient (10 min) leaching treatments with nutrient-free buffer underneath the membranes resulted in abnormal encystment and poor germination. The disruption was greatest when leaching was applied during the first minutes after start of encystment and not observed after 20 min. The early sensitivity of cells to leaching coincided with the period when alkali-resistant cell walls were formed (2-6 min after mechanical agitation). Effects of calcium and organic nutrients on encystment during leaching and germination after these treatments were studied. The disruption of encystment by early leaching treatments, but not the suppression of cyst germination, was overcome by adding calcium chloride during mechanical agitation of zoospores. Leaching with calcium containing buffer resulted in suppressed cyst germination as was the case with buffer alone. Leaching with 0.1% peptone containing buffer promoted consistently high encystment and germination.

    Originele taal-2Engels
    Pagina's (van-tot)235-43
    Aantal pagina's9
    TijdschriftAntonie Van Leeuwenhoek International Journal of General and Molecular Microbiology
    Volume83
    Nummer van het tijdschrift3
    StatusGepubliceerd - 2003

    Vingerafdruk

    Duik in de onderzoeksthema's van 'Defective zoospore encystment and suppressed cyst germination of Phytophthora palmivora caused by transient leaching treatments'. Samen vormen ze een unieke vingerafdruk.

    Citeer dit