Depicting the Discrepancy between Tri Genotype and Chemotype on the Basis of Strain CBS 139514 from a Field Population of F. graminearum Sensu Stricto from Argentina

Tomasz Kulik; Maciej Buśko; Katarzyna Bilska; Anna Ostrowska-Kołodziejczak; Anne D van Diepeningen; Juliusz Perkowski; Sebastian Stenglein

doi:10.3390/toxins8110330

Depicting the Discrepancy between Tri Genotype and Chemotype on the Basis of Strain CBS 139514 from a Field Population of F. graminearum Sensu Stricto from Argentina

Tomasz Kulik, Maciej Buśko, Katarzyna Bilska, Anna Ostrowska-Kołodziejczak, Anne D van Diepeningen, Juliusz Perkowski, Sebastian Stenglein

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

10 Citaten (Scopus)

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Recent studies on a field population of F. graminearum sensu stricto from Argentina revealed an atypical panel of strains identified through PCR genotyping as 15ADON genotypes, but producing high levels of 3ADON. Based on representative strain CBS 139514, we asked if the discrepancy between the trichothecene genotype and chemotype might result from an inter-chemotype recombination of the chemotype-determining genes. To answer this, we sequenced the complete core Tri gene cluster (around 30,200 bp) from this strain and compared its sequence to sequence data of typical type B trichothecene genotypes/chemotypes. Sequence alignment showed that CBS 139514 has an identical sequence within the entire core Tri cluster to the 15ADON genotype. The revealed discrepancy underlines the need for using both molecular and chemical methods for reliable characterization of toxigenic strains of Fusarium.

Originele taal-2	Engels
Tijdschrift	Toxins
Volume	8
Nummer van het tijdschrift	11
DOI's	https://doi.org/10.3390/toxins8110330
Status	Gepubliceerd - 12 nov. 2016

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@article{909bc93e71954449b33448c67a729315,

title = "Depicting the Discrepancy between Tri Genotype and Chemotype on the Basis of Strain CBS 139514 from a Field Population of F. graminearum Sensu Stricto from Argentina",

abstract = "Recent studies on a field population of F. graminearum sensu stricto from Argentina revealed an atypical panel of strains identified through PCR genotyping as 15ADON genotypes, but producing high levels of 3ADON. Based on representative strain CBS 139514, we asked if the discrepancy between the trichothecene genotype and chemotype might result from an inter-chemotype recombination of the chemotype-determining genes. To answer this, we sequenced the complete core Tri gene cluster (around 30,200 bp) from this strain and compared its sequence to sequence data of typical type B trichothecene genotypes/chemotypes. Sequence alignment showed that CBS 139514 has an identical sequence within the entire core Tri cluster to the 15ADON genotype. The revealed discrepancy underlines the need for using both molecular and chemical methods for reliable characterization of toxigenic strains of Fusarium.",

author = "Tomasz Kulik and Maciej Bu{\'s}ko and Katarzyna Bilska and Anna Ostrowska-Ko{\l}odziejczak and {van Diepeningen}, {Anne D} and Juliusz Perkowski and Sebastian Stenglein",

year = "2016",

month = nov,

day = "12",

doi = "10.3390/toxins8110330",

language = "English",

volume = "8",

journal = "Toxins",

issn = "2072-6651",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "11",

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Depicting the Discrepancy between Tri Genotype and Chemotype on the Basis of Strain CBS 139514 from a Field Population of F. graminearum Sensu Stricto from Argentina. / Kulik, Tomasz; Buśko, Maciej; Bilska, Katarzyna et al.
In: Toxins, Vol. 8, Nr. 11, 12.11.2016.

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

TY - JOUR

T1 - Depicting the Discrepancy between Tri Genotype and Chemotype on the Basis of Strain CBS 139514 from a Field Population of F. graminearum Sensu Stricto from Argentina

AU - Kulik, Tomasz

AU - Buśko, Maciej

AU - Bilska, Katarzyna

AU - Ostrowska-Kołodziejczak, Anna

AU - van Diepeningen, Anne D

AU - Perkowski, Juliusz

AU - Stenglein, Sebastian

PY - 2016/11/12

Y1 - 2016/11/12

N2 - Recent studies on a field population of F. graminearum sensu stricto from Argentina revealed an atypical panel of strains identified through PCR genotyping as 15ADON genotypes, but producing high levels of 3ADON. Based on representative strain CBS 139514, we asked if the discrepancy between the trichothecene genotype and chemotype might result from an inter-chemotype recombination of the chemotype-determining genes. To answer this, we sequenced the complete core Tri gene cluster (around 30,200 bp) from this strain and compared its sequence to sequence data of typical type B trichothecene genotypes/chemotypes. Sequence alignment showed that CBS 139514 has an identical sequence within the entire core Tri cluster to the 15ADON genotype. The revealed discrepancy underlines the need for using both molecular and chemical methods for reliable characterization of toxigenic strains of Fusarium.

AB - Recent studies on a field population of F. graminearum sensu stricto from Argentina revealed an atypical panel of strains identified through PCR genotyping as 15ADON genotypes, but producing high levels of 3ADON. Based on representative strain CBS 139514, we asked if the discrepancy between the trichothecene genotype and chemotype might result from an inter-chemotype recombination of the chemotype-determining genes. To answer this, we sequenced the complete core Tri gene cluster (around 30,200 bp) from this strain and compared its sequence to sequence data of typical type B trichothecene genotypes/chemotypes. Sequence alignment showed that CBS 139514 has an identical sequence within the entire core Tri cluster to the 15ADON genotype. The revealed discrepancy underlines the need for using both molecular and chemical methods for reliable characterization of toxigenic strains of Fusarium.

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DO - 10.3390/toxins8110330

M3 - Article

C2 - 27845742

SN - 2072-6651

VL - 8

JO - Toxins

JF - Toxins

IS - 11

ER -

Depicting the Discrepancy between Tri Genotype and Chemotype on the Basis of Strain CBS 139514 from a Field Population of F. graminearum Sensu Stricto from Argentina

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