Deriving cell lines from zebrafish embryos and tumors

S. Choorapoikayil; J. Overvoorde; J. den Hertog

doi:10.1089/zeb.2013.0866

Deriving cell lines from zebrafish embryos and tumors

S. Choorapoikayil, J. Overvoorde, J. den Hertog

Hubrecht Institute

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

22 Citaten (Scopus)

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Over the last two decades the zebrafish has emerged as a powerful model organism in science. The experimental accessibility, the broad range of zebrafish mutants, and the highly conserved genetic and biochemical pathways between zebrafish and mammals lifted zebrafish to become one of the most attractive vertebrate models to study gene function and to model human diseases. Zebrafish cell lines are highly attractive to investigate cell biology and zebrafish cell lines complement the experimental tools that are available already. We established a straightforward method to culture cells from a single zebrafish embryo or a single tumor. Here we describe the generation of fibroblast-like cell lines from wild-type and ptenb(-/-) embryos and an endothelial-like cell line from a tumor of an adult ptena(+/-)ptenb(-/-) zebrafish. This protocol can easily be adapted to establish stable cell lines from any mutant or transgenic zebrafish line and the average time to obtain a pro-stable cell line is 3-5 months.

Originele taal-2	Engels
Pagina's (van-tot)	316-325
Tijdschrift	Zebrafish
Volume	10
Nummer van het tijdschrift	3
DOI's	https://doi.org/10.1089/zeb.2013.0866
Status	Gepubliceerd - 2013

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author = "S. Choorapoikayil and J. Overvoorde and {den Hertog}, J.",

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Deriving cell lines from zebrafish embryos and tumors

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