TY - JOUR
T1 - Detection and quantification of the histone code in the fungal genus Aspergillus
AU - Zhang, Xin
AU - Noberini, Roberta
AU - Vai, Alessandro
AU - Bonaldi, Tiziana
AU - Seidl, Michael F.
AU - Collemare, Jérȏme
N1 - Funding Information:
Xin Zhang is funded by the Chinese Scholarship Council (CSC) (201907720028). This work was supported by EPIC-XS, project number 823 839, funded by the Horizon 2020 programme of the European Union.
Publisher Copyright:
© 2023 The Author(s)
PY - 2023/6
Y1 - 2023/6
N2 - In eukaryotes, the combination of different histone post-translational modifications (PTMs) – the histone code – impacts the chromatin organization as compact and transcriptionally silent heterochromatin or accessible and transcriptionally active euchromatin. Although specific histone PTMs have been studied in fungi, an overview of histone PTMs and their relative abundance is still lacking. Here, we used mass spectrometry to detect and quantify histone PTMs in three fungal species belonging to three distinct taxonomic sections of the genus Aspergillus (Aspergillus niger, Aspergillus nidulans (two strains), and Aspergillus fumigatus). We overall detected 23 different histone PTMs, including a majority of lysine methylations and acetylations, and 23 co-occurrence patterns of multiple histone PTMs. Among those, we report for the first time the detection of H3K79me1, H3K79me2, and H4K31ac in Aspergilli. Although all three species harbour the same PTMs, we found significant differences in the relative abundance of H3K9me1/2/3, H3K14ac, H3K36me1 and H3K79me1, as well as the co-occurrence of acetylation on both K18 and K23 of histone H3 in a strain-specific manner. Our results provide novel insights about the underexplored complexity of the histone code in filamentous fungi, and its functional implications on genome architecture and gene regulation.
AB - In eukaryotes, the combination of different histone post-translational modifications (PTMs) – the histone code – impacts the chromatin organization as compact and transcriptionally silent heterochromatin or accessible and transcriptionally active euchromatin. Although specific histone PTMs have been studied in fungi, an overview of histone PTMs and their relative abundance is still lacking. Here, we used mass spectrometry to detect and quantify histone PTMs in three fungal species belonging to three distinct taxonomic sections of the genus Aspergillus (Aspergillus niger, Aspergillus nidulans (two strains), and Aspergillus fumigatus). We overall detected 23 different histone PTMs, including a majority of lysine methylations and acetylations, and 23 co-occurrence patterns of multiple histone PTMs. Among those, we report for the first time the detection of H3K79me1, H3K79me2, and H4K31ac in Aspergilli. Although all three species harbour the same PTMs, we found significant differences in the relative abundance of H3K9me1/2/3, H3K14ac, H3K36me1 and H3K79me1, as well as the co-occurrence of acetylation on both K18 and K23 of histone H3 in a strain-specific manner. Our results provide novel insights about the underexplored complexity of the histone code in filamentous fungi, and its functional implications on genome architecture and gene regulation.
KW - Aspergillus fumigatus
KW - Aspergillus nidulans
KW - Aspergillus niger
KW - Histone acetylation
KW - Histone extraction
KW - Histone methylation
KW - Mass spectrometry
UR - http://www.scopus.com/inward/record.url?scp=85158893344&partnerID=8YFLogxK
U2 - 10.1016/j.fgb.2023.103800
DO - 10.1016/j.fgb.2023.103800
M3 - Article
C2 - 37146898
AN - SCOPUS:85158893344
SN - 1087-1845
VL - 167
JO - Fungal Genetics and Biology
JF - Fungal Genetics and Biology
M1 - 103800
ER -