Development and characterization of microsatellite markers for two dioecious Ficus species

M. Zavodna, P. Arens, P.J. van Dijk, B. Vosman

    Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgaveArtikelWetenschappelijkpeer review

    Samenvatting

    Microsatellite markers for Ficus montana and Ficus septica were developed using genomic libraries enriched for di-, tri- and tetranucleotide repeats. The subsets of five and three best scorable primer pairs were characterized on 24 F. montana and 36 F. septica individuals, respectively. For F. montana, loci showed five to 14 alleles per locus and expected heterozygosities ranged between 0.23 and 0.87. For F. septica, loci showed three to five alleles per locus and expected heterozygosities ranged between 0.36 and 0.49. Four primer pairs (two from each subset) cross-amplified in the other species, indicating transportability of the markers within the genus Ficus. [KEYWORDS: dispersal ; Ficus montana ; Ficus septica ; mutualism ; pollinating fig wasps ; simple sequence repeat (SSR)]
    Originele taal-2Engels
    Pagina's (van-tot)355-357
    TijdschriftMolecular Ecology Notes
    Volume5
    Nummer van het tijdschrift2
    DOI's
    StatusGepubliceerd - 2005

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