Development and characterization of microsatellite markers for two dioecious Ficus species

M. Zavodna; P. Arens; P.J. van Dijk; B. Vosman

doi:10.1111/j.1471-8286.2005.00924.x

Development and characterization of microsatellite markers for two dioecious Ficus species

M. Zavodna
, P. Arens
, P.J. van Dijk
, B. Vosman

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

36 Citaten (Scopus)

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Microsatellite markers for Ficus montana and Ficus septica were developed using genomic libraries enriched for di-, tri- and tetranucleotide repeats. The subsets of five and three best scorable primer pairs were characterized on 24 F. montana and 36 F. septica individuals, respectively. For F. montana, loci showed five to 14 alleles per locus and expected heterozygosities ranged between 0.23 and 0.87. For F. septica, loci showed three to five alleles per locus and expected heterozygosities ranged between 0.36 and 0.49. Four primer pairs (two from each subset) cross-amplified in the other species, indicating transportability of the markers within the genus Ficus. [KEYWORDS: dispersal ; Ficus montana ; Ficus septica ; mutualism ; pollinating fig wasps ; simple sequence repeat (SSR)]

Originele taal-2	Engels
Pagina's (van-tot)	355-357
Tijdschrift	Molecular Ecology Notes
Volume	5
Nummer van het tijdschrift	2
DOI's	https://doi.org/10.1111/j.1471-8286.2005.00924.x
Status	Gepubliceerd - 2005

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title = "Development and characterization of microsatellite markers for two dioecious Ficus species",

abstract = "Microsatellite markers for Ficus montana and Ficus septica were developed using genomic libraries enriched for di-, tri- and tetranucleotide repeats. The subsets of five and three best scorable primer pairs were characterized on 24 F. montana and 36 F. septica individuals, respectively. For F. montana, loci showed five to 14 alleles per locus and expected heterozygosities ranged between 0.23 and 0.87. For F. septica, loci showed three to five alleles per locus and expected heterozygosities ranged between 0.36 and 0.49. Four primer pairs (two from each subset) cross-amplified in the other species, indicating transportability of the markers within the genus Ficus. [KEYWORDS: dispersal ; Ficus montana ; Ficus septica ; mutualism ; pollinating fig wasps ; simple sequence repeat (SSR)]",

author = "M. Zavodna and P. Arens and \{van Dijk\}, P.J. and B. Vosman",

note = "Reporting year: 2005 Metis note: 3578; CTE; PVP ; TE file:///L:/Endnotedatabases/NIOOPUB/pdfs/Pdfs2005/Zavodna\_ea\_3578.pdf",

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doi = "10.1111/j.1471-8286.2005.00924.x",

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AU - Zavodna, M.

AU - Arens, P.

AU - van Dijk, P.J.

AU - Vosman, B.

N1 - Reporting year: 2005 Metis note: 3578; CTE; PVP ; TE file:///L:/Endnotedatabases/NIOOPUB/pdfs/Pdfs2005/Zavodna_ea_3578.pdf

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AB - Microsatellite markers for Ficus montana and Ficus septica were developed using genomic libraries enriched for di-, tri- and tetranucleotide repeats. The subsets of five and three best scorable primer pairs were characterized on 24 F. montana and 36 F. septica individuals, respectively. For F. montana, loci showed five to 14 alleles per locus and expected heterozygosities ranged between 0.23 and 0.87. For F. septica, loci showed three to five alleles per locus and expected heterozygosities ranged between 0.36 and 0.49. Four primer pairs (two from each subset) cross-amplified in the other species, indicating transportability of the markers within the genus Ficus. [KEYWORDS: dispersal ; Ficus montana ; Ficus septica ; mutualism ; pollinating fig wasps ; simple sequence repeat (SSR)]

U2 - 10.1111/j.1471-8286.2005.00924.x

DO - 10.1111/j.1471-8286.2005.00924.x

M3 - Article

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VL - 5

SP - 355

EP - 357

JO - Molecular Ecology Notes

JF - Molecular Ecology Notes

IS - 2

ER -

Development and characterization of microsatellite markers for two dioecious Ficus species

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