Development of whole-soil microbial inoculants based on solid-phase fermentation for the regeneration of the functioning of vineyard soils

Juan F. Aguiar; Isabel M. Liberal; María L. Muñoz; Emma Cantos-Villar; Belén Puertas; Antonio Archidona-Yuste; Ramón Casimiro-Soriguer; Martina Coletta; Aldo D'Alessandro; Ana González-Robles; David Gramaje; Delphine Renard; Mélanie Roy; Antonietta La Terza; Rubén Tarifa; Corinne Vacher; G. F.(Ciska) Veen; Raúl Ochoa-Hueso

doi:10.1016/j.rhisph.2025.101039

Development of whole-soil microbial inoculants based on solid-phase fermentation for the regeneration of the functioning of vineyard soils

Juan F. Aguiar^* (Co-auteur), Isabel M. Liberal, María L. Muñoz, Emma Cantos-Villar, Belén Puertas, Antonio Archidona-Yuste, Ramón Casimiro-Soriguer, Martina Coletta, Aldo D'Alessandro, Ana González-Robles, David Gramaje, Delphine Renard, Mélanie Roy, Antonietta La Terza, Rubén Tarifa, Corinne Vacher, G. F.(Ciska) Veen, Raúl Ochoa-Hueso^* (Co-auteur)

^*Corresponding author voor dit werk

NIOO - Terrestrial Ecology (TE)

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

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Achieving the sustainability of modern agriculture will require, among other actions, an improvement of the soil and its associated microbiota. One way to achieve this is through the inoculation with beneficial soil microbial communities. In this study, we used solid-phase fermentations to produce 25 distinct microbial inoculants based on complex communities obtained from the rhizosphere of 23 European vineyards. For this purpose, we mixed 0.1 g of donor rhizosphere soil and 25 g of ground and sterilized growing substrate composed of winemaking byproducts in 15-cm Petri dishes. Aerobic fermentations were carried out for a period of 56 days and the activity of microbial enzymes linked to the biogeochemical cycling of carbon, nitrogen, phosphorus, and sulfur was evaluated every two weeks. We then carried out a common garden experiment where the inoculants were tested in pots containing vineyard soil and planted with Tempranillo vines. During the fermentation, the enzyme activity of the inoculants evolved from no activity to high-activity values. Carbon- and phosphorus-linked enzymes tended to show higher activity after 14 days of incubation and then decreased or remained constant, while nitrogen-linked enzymes tended to show their highest values after 28 days of incubation. Despite these general patterns, inoculants developed from different rhizosphere communities followed different trajectories in terms of activity. In addition, we observed significant relationships between the enzyme activity of donor rhizosphere soils and the enzyme activity of inoculants, especially after 28 days of incubation. We also found a significant relationship between the enzyme activity of the inoculants and the enzyme activity measured in the soil of pots containing vines. Our results suggest the possibility of predicting the metabolic potential of the inoculants from the metabolic potential of their donor soil sample, as well as the possibility of transferring these metabolic capabilities to soils, with likely applications for the regeneration of the functioning of vineyards.

Originele taal-2	Engels
Artikelnummer	101039
Tijdschrift	Rhizosphere
Volume	34
Vroegere onlinedatum	19 mrt. 2025
DOI's	https://doi.org/10.1016/j.rhisph.2025.101039
Status	Gepubliceerd - jun. 2025

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10.1016/j.rhisph.2025.101039

Aguiar et al 2025Final published version, 8,95 MBLicentie: Taverne

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Aguiar, J. F., Liberal, I. M., Muñoz, M. L., Cantos-Villar, E., Puertas, B., Archidona-Yuste, A., Casimiro-Soriguer, R., Coletta, M., D'Alessandro, A., González-Robles, A., Gramaje, D., Renard, D., Roy, M., La Terza, A., Tarifa, R., Vacher, C., Veen, G. F., & Ochoa-Hueso, R. (2025). Development of whole-soil microbial inoculants based on solid-phase fermentation for the regeneration of the functioning of vineyard soils. Rhizosphere, 34, Artikel 101039. https://doi.org/10.1016/j.rhisph.2025.101039

@article{5d167a124b4241579bdd6f963b904561,

title = "Development of whole-soil microbial inoculants based on solid-phase fermentation for the regeneration of the functioning of vineyard soils",

abstract = "Achieving the sustainability of modern agriculture will require, among other actions, an improvement of the soil and its associated microbiota. One way to achieve this is through the inoculation with beneficial soil microbial communities. In this study, we used solid-phase fermentations to produce 25 distinct microbial inoculants based on complex communities obtained from the rhizosphere of 23 European vineyards. For this purpose, we mixed 0.1 g of donor rhizosphere soil and 25 g of ground and sterilized growing substrate composed of winemaking byproducts in 15-cm Petri dishes. Aerobic fermentations were carried out for a period of 56 days and the activity of microbial enzymes linked to the biogeochemical cycling of carbon, nitrogen, phosphorus, and sulfur was evaluated every two weeks. We then carried out a common garden experiment where the inoculants were tested in pots containing vineyard soil and planted with Tempranillo vines. During the fermentation, the enzyme activity of the inoculants evolved from no activity to high-activity values. Carbon- and phosphorus-linked enzymes tended to show higher activity after 14 days of incubation and then decreased or remained constant, while nitrogen-linked enzymes tended to show their highest values after 28 days of incubation. Despite these general patterns, inoculants developed from different rhizosphere communities followed different trajectories in terms of activity. In addition, we observed significant relationships between the enzyme activity of donor rhizosphere soils and the enzyme activity of inoculants, especially after 28 days of incubation. We also found a significant relationship between the enzyme activity of the inoculants and the enzyme activity measured in the soil of pots containing vines. Our results suggest the possibility of predicting the metabolic potential of the inoculants from the metabolic potential of their donor soil sample, as well as the possibility of transferring these metabolic capabilities to soils, with likely applications for the regeneration of the functioning of vineyards.",

keywords = "Biofertilizer, Enzyme activity, Inoculum, Rhizosphere, Soil microorganisms, Solid-phase fermentation",

author = "Aguiar, {Juan F.} and Liberal, {Isabel M.} and Mu{\~n}oz, {Mar{\'i}a L.} and Emma Cantos-Villar and Bel{\'e}n Puertas and Antonio Archidona-Yuste and Ram{\'o}n Casimiro-Soriguer and Martina Coletta and Aldo D'Alessandro and Ana Gonz{\'a}lez-Robles and David Gramaje and Delphine Renard and M{\'e}lanie Roy and {La Terza}, Antonietta and Rub{\'e}n Tarifa and Corinne Vacher and Veen, {G. F.(Ciska)} and Ra{\'u}l Ochoa-Hueso",

note = "Data archiving: Confidential data",

year = "2025",

month = jun,

doi = "10.1016/j.rhisph.2025.101039",

language = "English",

volume = "34",

journal = "Rhizosphere",

issn = "2452-2198",

publisher = "Elsevier B.V.",

}

Aguiar, JF, Liberal, IM, Muñoz, ML, Cantos-Villar, E, Puertas, B, Archidona-Yuste, A, Casimiro-Soriguer, R, Coletta, M, D'Alessandro, A, González-Robles, A, Gramaje, D, Renard, D, Roy, M, La Terza, A, Tarifa, R, Vacher, C, Veen, GF & Ochoa-Hueso, R 2025, 'Development of whole-soil microbial inoculants based on solid-phase fermentation for the regeneration of the functioning of vineyard soils', Rhizosphere, vol. 34, 101039. https://doi.org/10.1016/j.rhisph.2025.101039

Development of whole-soil microbial inoculants based on solid-phase fermentation for the regeneration of the functioning of vineyard soils. / Aguiar, Juan F. (Co-auteur); Liberal, Isabel M.; Muñoz, María L. et al.
In: Rhizosphere, Vol. 34, 101039, 06.2025.

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

TY - JOUR

T1 - Development of whole-soil microbial inoculants based on solid-phase fermentation for the regeneration of the functioning of vineyard soils

AU - Aguiar, Juan F.

AU - Liberal, Isabel M.

AU - Muñoz, María L.

AU - Cantos-Villar, Emma

AU - Puertas, Belén

AU - Archidona-Yuste, Antonio

AU - Casimiro-Soriguer, Ramón

AU - Coletta, Martina

AU - D'Alessandro, Aldo

AU - González-Robles, Ana

AU - Gramaje, David

AU - Renard, Delphine

AU - Roy, Mélanie

AU - La Terza, Antonietta

AU - Tarifa, Rubén

AU - Vacher, Corinne

AU - Veen, G. F.(Ciska)

AU - Ochoa-Hueso, Raúl

N1 - Data archiving: Confidential data

PY - 2025/6

Y1 - 2025/6

N2 - Achieving the sustainability of modern agriculture will require, among other actions, an improvement of the soil and its associated microbiota. One way to achieve this is through the inoculation with beneficial soil microbial communities. In this study, we used solid-phase fermentations to produce 25 distinct microbial inoculants based on complex communities obtained from the rhizosphere of 23 European vineyards. For this purpose, we mixed 0.1 g of donor rhizosphere soil and 25 g of ground and sterilized growing substrate composed of winemaking byproducts in 15-cm Petri dishes. Aerobic fermentations were carried out for a period of 56 days and the activity of microbial enzymes linked to the biogeochemical cycling of carbon, nitrogen, phosphorus, and sulfur was evaluated every two weeks. We then carried out a common garden experiment where the inoculants were tested in pots containing vineyard soil and planted with Tempranillo vines. During the fermentation, the enzyme activity of the inoculants evolved from no activity to high-activity values. Carbon- and phosphorus-linked enzymes tended to show higher activity after 14 days of incubation and then decreased or remained constant, while nitrogen-linked enzymes tended to show their highest values after 28 days of incubation. Despite these general patterns, inoculants developed from different rhizosphere communities followed different trajectories in terms of activity. In addition, we observed significant relationships between the enzyme activity of donor rhizosphere soils and the enzyme activity of inoculants, especially after 28 days of incubation. We also found a significant relationship between the enzyme activity of the inoculants and the enzyme activity measured in the soil of pots containing vines. Our results suggest the possibility of predicting the metabolic potential of the inoculants from the metabolic potential of their donor soil sample, as well as the possibility of transferring these metabolic capabilities to soils, with likely applications for the regeneration of the functioning of vineyards.

AB - Achieving the sustainability of modern agriculture will require, among other actions, an improvement of the soil and its associated microbiota. One way to achieve this is through the inoculation with beneficial soil microbial communities. In this study, we used solid-phase fermentations to produce 25 distinct microbial inoculants based on complex communities obtained from the rhizosphere of 23 European vineyards. For this purpose, we mixed 0.1 g of donor rhizosphere soil and 25 g of ground and sterilized growing substrate composed of winemaking byproducts in 15-cm Petri dishes. Aerobic fermentations were carried out for a period of 56 days and the activity of microbial enzymes linked to the biogeochemical cycling of carbon, nitrogen, phosphorus, and sulfur was evaluated every two weeks. We then carried out a common garden experiment where the inoculants were tested in pots containing vineyard soil and planted with Tempranillo vines. During the fermentation, the enzyme activity of the inoculants evolved from no activity to high-activity values. Carbon- and phosphorus-linked enzymes tended to show higher activity after 14 days of incubation and then decreased or remained constant, while nitrogen-linked enzymes tended to show their highest values after 28 days of incubation. Despite these general patterns, inoculants developed from different rhizosphere communities followed different trajectories in terms of activity. In addition, we observed significant relationships between the enzyme activity of donor rhizosphere soils and the enzyme activity of inoculants, especially after 28 days of incubation. We also found a significant relationship between the enzyme activity of the inoculants and the enzyme activity measured in the soil of pots containing vines. Our results suggest the possibility of predicting the metabolic potential of the inoculants from the metabolic potential of their donor soil sample, as well as the possibility of transferring these metabolic capabilities to soils, with likely applications for the regeneration of the functioning of vineyards.

KW - Biofertilizer

KW - Enzyme activity

KW - Inoculum

KW - Rhizosphere

KW - Soil microorganisms

KW - Solid-phase fermentation

U2 - 10.1016/j.rhisph.2025.101039

DO - 10.1016/j.rhisph.2025.101039

M3 - Article

AN - SCOPUS:105000375579

SN - 2452-2198

VL - 34

JO - Rhizosphere

JF - Rhizosphere

M1 - 101039

ER -

Development of whole-soil microbial inoculants based on solid-phase fermentation for the regeneration of the functioning of vineyard soils

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