Differential Stoichiometry among Core Ribosomal Proteins

Nikolai Slavov, Stefan Semrau, Edoardo Airoldi, Bogdan Budnik, Alexander van Oudenaarden

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgaveArtikelWetenschappelijkpeer review

122 Citaten (Scopus)


Understanding the regulation and structure of ribosomes is essential to understanding protein synthesis and its dysregulation in disease. While ribosomes are believed to have a fixed stoichiometry among their core ribosomal proteins (RPs), some experiments suggest a more variable composition. Testing such variability requires direct and precise quantification of RPs. We used mass spectrometry to directly quantify RPs across monosomes and polysomes of mouse embryonic stem cells (ESC) and budding yeast. Our data show that the stoichiometry among core RPs in wild-type yeast cells and ESC depends both on the growth conditions and on the number of ribosomes bound per mRNA. Furthermore, we find that the fitness of cells with a deleted RP-gene is inversely proportional to the enrichment of the corresponding RP in polysomes. Together, our findings support the existence of ribosomes with distinct protein composition and physiological function.

Originele taal-2Engels
Pagina's (van-tot)865-73
Aantal pagina's9
TijdschriftCell Reports
Nummer van het tijdschrift5
StatusGepubliceerd - 03 nov. 2015


Duik in de onderzoeksthema's van 'Differential Stoichiometry among Core Ribosomal Proteins'. Samen vormen ze een unieke vingerafdruk.

Citeer dit