Discovery of Thanafactin A, a Linear Proline-containing Octa-Lipopeptide from Pseudomonas sp. SH-C52, Motivated by Genome Mining

Norbert Kirchner, Carolina Cano-Prieto, Anna Schulz-Finke, M. Gütschow, Nico Ortlieb, Julia Moschny, Timo Niedermeyer, Jeannie Horak, Michael Lämmerhofer, Menno van der Voort, J.M. Raaijmakers, Harald Gross (Co-auteur)

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgaveArtikelWetenschappelijkpeer review

Samenvatting

Genome mining of the bacterial strains Pseudomonas sp. SH-C52 and Pseudomonas fluorescens DSM 11579 showed that both strains contained a highly similar gene cluster encoding an octamodular nonribosomal peptide synthetase (NRPS) system which was not associated with a known secondary metabolite. Insertional mutagenesis of an NRPS component followed by comparative profiling led to the discovery of the corresponding novel linear octalipopeptide thanafactin A, which was subsequently isolated and its structure determined by two-dimensional NMR and further spectroscopic and chromatographic methods. In bioassays, thanafactin A exhibited weak protease inhibitory activity and was found to modulate swarming motility in a strain-specific manner.
Originele taal-2Engels
Pagina's (van-tot)101–109
TijdschriftJournal of Natural Products
Volume84
Nummer van het tijdschrift1
DOI's
StatusGepubliceerd - jan 2021

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