Discovery of Thanafactin A, a Linear Proline-containing Octa-Lipopeptide from Pseudomonas sp. SH-C52, Motivated by Genome Mining

Norbert Kirchner; Carolina Cano-Prieto; Anna Schulz-Finke; M. Gütschow; Nico Ortlieb; Julia Moschny; Timo Niedermeyer; Jeannie Horak; Michael Lämmerhofer; Menno van der Voort; J.M. Raaijmakers; Harald Gross

doi:10.1021/acs.jnatprod.0c01174

Discovery of Thanafactin A, a Linear Proline-containing Octa-Lipopeptide from Pseudomonas sp. SH-C52, Motivated by Genome Mining

Norbert Kirchner, Carolina Cano-Prieto, Anna Schulz-Finke, M. Gütschow, Nico Ortlieb, Julia Moschny, Timo Niedermeyer, Jeannie Horak, Michael Lämmerhofer, Menno van der Voort, J.M. Raaijmakers, Harald Gross (Co-auteur)

NIOO - Microbial Ecology (ME)

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

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Genome mining of the bacterial strains Pseudomonas sp. SH-C52 and Pseudomonas fluorescens DSM 11579 showed that both strains contained a highly similar gene cluster encoding an octamodular nonribosomal peptide synthetase (NRPS) system which was not associated with a known secondary metabolite. Insertional mutagenesis of an NRPS component followed by comparative profiling led to the discovery of the corresponding novel linear octalipopeptide thanafactin A, which was subsequently isolated and its structure determined by two-dimensional NMR and further spectroscopic and chromatographic methods. In bioassays, thanafactin A exhibited weak protease inhibitory activity and was found to modulate swarming motility in a strain-specific manner.

Originele taal-2	Engels
Pagina's (van-tot)	101–109
Aantal pagina's	9
Tijdschrift	Journal of Natural Products
Volume	84
Nummer van het tijdschrift	1
DOI's	https://doi.org/10.1021/acs.jnatprod.0c01174
Status	Gepubliceerd - 2021

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10.1021/acs.jnatprod.0c01174

Kirchner et al 2021 AAMAccepted author manuscript, 1,58 MB

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Kirchner, N., Cano-Prieto, C., Schulz-Finke, A., Gütschow, M., Ortlieb, N., Moschny, J., Niedermeyer, T., Horak, J., Lämmerhofer, M., van der Voort, M., Raaijmakers, J. M., & Gross, H. (2021). Discovery of Thanafactin A, a Linear Proline-containing Octa-Lipopeptide from Pseudomonas sp. SH-C52, Motivated by Genome Mining. Journal of Natural Products, 84(1), 101–109. https://doi.org/10.1021/acs.jnatprod.0c01174

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title = "Discovery of Thanafactin A, a Linear Proline-containing Octa-Lipopeptide from Pseudomonas sp. SH-C52, Motivated by Genome Mining",

abstract = "Genome mining of the bacterial strains Pseudomonas sp. SH-C52 and Pseudomonas fluorescens DSM 11579 showed that both strains contained a highly similar gene cluster encoding an octamodular nonribosomal peptide synthetase (NRPS) system which was not associated with a known secondary metabolite. Insertional mutagenesis of an NRPS component followed by comparative profiling led to the discovery of the corresponding novel linear octalipopeptide thanafactin A, which was subsequently isolated and its structure determined by two-dimensional NMR and further spectroscopic and chromatographic methods. In bioassays, thanafactin A exhibited weak protease inhibitory activity and was found to modulate swarming motility in a strain-specific manner.",

keywords = "International, Plan_S-Compliant_NO",

author = "Norbert Kirchner and Carolina Cano-Prieto and Anna Schulz-Finke and M. G{\"u}tschow and Nico Ortlieb and Julia Moschny and Timo Niedermeyer and Jeannie Horak and Michael L{\"a}mmerhofer and {van der Voort}, Menno and J.M. Raaijmakers and Harald Gross",

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year = "2021",

doi = "10.1021/acs.jnatprod.0c01174",

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pages = "101–109",

journal = "Journal of Natural Products",

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Kirchner, N, Cano-Prieto, C, Schulz-Finke, A, Gütschow, M, Ortlieb, N, Moschny, J, Niedermeyer, T, Horak, J, Lämmerhofer, M, van der Voort, M, Raaijmakers, JM & Gross, H 2021, 'Discovery of Thanafactin A, a Linear Proline-containing Octa-Lipopeptide from Pseudomonas sp. SH-C52, Motivated by Genome Mining', Journal of Natural Products, vol. 84, nr. 1, blz. 101–109. https://doi.org/10.1021/acs.jnatprod.0c01174

Discovery of Thanafactin A, a Linear Proline-containing Octa-Lipopeptide from Pseudomonas sp. SH-C52, Motivated by Genome Mining. / Kirchner, Norbert; Cano-Prieto, Carolina; Schulz-Finke, Anna et al.
In: Journal of Natural Products, Vol. 84, Nr. 1, 2021, blz. 101–109.

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

TY - JOUR

T1 - Discovery of Thanafactin A, a Linear Proline-containing Octa-Lipopeptide from Pseudomonas sp. SH-C52, Motivated by Genome Mining

AU - Kirchner, Norbert

AU - Cano-Prieto, Carolina

AU - Schulz-Finke, Anna

AU - Gütschow, M.

AU - Ortlieb, Nico

AU - Moschny, Julia

AU - Niedermeyer, Timo

AU - Horak, Jeannie

AU - Lämmerhofer, Michael

AU - van der Voort, Menno

AU - Raaijmakers, J.M.

AU - Gross, Harald

N1 - 7118, ME; Data archiving: not NIOO data

PY - 2021

Y1 - 2021

N2 - Genome mining of the bacterial strains Pseudomonas sp. SH-C52 and Pseudomonas fluorescens DSM 11579 showed that both strains contained a highly similar gene cluster encoding an octamodular nonribosomal peptide synthetase (NRPS) system which was not associated with a known secondary metabolite. Insertional mutagenesis of an NRPS component followed by comparative profiling led to the discovery of the corresponding novel linear octalipopeptide thanafactin A, which was subsequently isolated and its structure determined by two-dimensional NMR and further spectroscopic and chromatographic methods. In bioassays, thanafactin A exhibited weak protease inhibitory activity and was found to modulate swarming motility in a strain-specific manner.

AB - Genome mining of the bacterial strains Pseudomonas sp. SH-C52 and Pseudomonas fluorescens DSM 11579 showed that both strains contained a highly similar gene cluster encoding an octamodular nonribosomal peptide synthetase (NRPS) system which was not associated with a known secondary metabolite. Insertional mutagenesis of an NRPS component followed by comparative profiling led to the discovery of the corresponding novel linear octalipopeptide thanafactin A, which was subsequently isolated and its structure determined by two-dimensional NMR and further spectroscopic and chromatographic methods. In bioassays, thanafactin A exhibited weak protease inhibitory activity and was found to modulate swarming motility in a strain-specific manner.

KW - International

KW - Plan_S-Compliant_NO

U2 - 10.1021/acs.jnatprod.0c01174

DO - 10.1021/acs.jnatprod.0c01174

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VL - 84

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JO - Journal of Natural Products

JF - Journal of Natural Products

IS - 1

ER -

Discovery of Thanafactin A, a Linear Proline-containing Octa-Lipopeptide from Pseudomonas sp. SH-C52, Motivated by Genome Mining

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