Distinct functions for ERK1 and ERK2 in cell migration processes during zebrafish gastrulation.

S.F.G. Krens; S. He; G.E.M. Lamers; A.H. Meijer; J. Bakkers; T. Schmidt; H.P. Spaink; B.E. Snaar-Jagalska

doi:10.1016/j.ydbio.2008.04.032

Distinct functions for ERK1 and ERK2 in cell migration processes during zebrafish gastrulation.

S.F.G. Krens, S. He, G.E.M. Lamers, A.H. Meijer, J. Bakkers, T. Schmidt, H.P. Spaink, B.E. Snaar-Jagalska

Hubrecht Institute

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

58 Citaten (Scopus)

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The MAPKs are key regulatory signaling molecules in many cellular processes. Here we define differential functions for ERK1 and ERK2 MAPKs in zebrafish embryogenesis. Morpholino knockdown of ERK1 and ERK2 resulted in cell migration defects during gastrulation, which could be rescued by co-injection of the corresponding mRNA. Strikingly, Erk2 mRNA cross-rescued ERK1 knockdown, but erk1 mRNA was unable to compensate for ERK2 knockdown. Cell-tracing experiments revealed a convergence defect for ERK1 morphants without a severe posterior-extension defect, whereas ERK2 morphants showed a more severe reduction in anterior-posterior extension. These defects were primary changes in gastrulation cell movements and not caused by altered cell fate specification. Saturating knockdown conditions showed that the absence of FGF-mediated dual-phosphorylated ERK2 from the blastula margin blocked initiation of epiboly, actin and tubulin cytoskeleton reorganization processes and further arrested embryogenesis, whereas ERK1 knockdown had only a mild effect on epiboly progression. Together, our data define distinct roles for ERK1 and ERK2 in developmental cell migration processes during zebrafish embryogenesis.

Originele taal-2	Engels
Pagina's (van-tot)	370-383
Tijdschrift	Developmental Biology
Volume	319
Nummer van het tijdschrift	2
DOI's	https://doi.org/10.1016/j.ydbio.2008.04.032
Status	Gepubliceerd - 2008

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10.1016/j.ydbio.2008.04.032

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@article{14b29d6b5cd3465f94c07ea48e1a4e26,

title = "Distinct functions for ERK1 and ERK2 in cell migration processes during zebrafish gastrulation.",

abstract = "The MAPKs are key regulatory signaling molecules in many cellular processes. Here we define differential functions for ERK1 and ERK2 MAPKs in zebrafish embryogenesis. Morpholino knockdown of ERK1 and ERK2 resulted in cell migration defects during gastrulation, which could be rescued by co-injection of the corresponding mRNA. Strikingly, Erk2 mRNA cross-rescued ERK1 knockdown, but erk1 mRNA was unable to compensate for ERK2 knockdown. Cell-tracing experiments revealed a convergence defect for ERK1 morphants without a severe posterior-extension defect, whereas ERK2 morphants showed a more severe reduction in anterior-posterior extension. These defects were primary changes in gastrulation cell movements and not caused by altered cell fate specification. Saturating knockdown conditions showed that the absence of FGF-mediated dual-phosphorylated ERK2 from the blastula margin blocked initiation of epiboly, actin and tubulin cytoskeleton reorganization processes and further arrested embryogenesis, whereas ERK1 knockdown had only a mild effect on epiboly progression. Together, our data define distinct roles for ERK1 and ERK2 in developmental cell migration processes during zebrafish embryogenesis.",

author = "S.F.G. Krens and S. He and G.E.M. Lamers and A.H. Meijer and J. Bakkers and T. Schmidt and H.P. Spaink and B.E. Snaar-Jagalska",

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T1 - Distinct functions for ERK1 and ERK2 in cell migration processes during zebrafish gastrulation.

AU - Krens, S.F.G.

AU - He, S.

AU - Lamers, G.E.M.

AU - Meijer, A.H.

AU - Bakkers, J.

AU - Schmidt, T.

AU - Spaink, H.P.

AU - Snaar-Jagalska, B.E.

N1 - Reporting year: 2008

PY - 2008

Y1 - 2008

N2 - The MAPKs are key regulatory signaling molecules in many cellular processes. Here we define differential functions for ERK1 and ERK2 MAPKs in zebrafish embryogenesis. Morpholino knockdown of ERK1 and ERK2 resulted in cell migration defects during gastrulation, which could be rescued by co-injection of the corresponding mRNA. Strikingly, Erk2 mRNA cross-rescued ERK1 knockdown, but erk1 mRNA was unable to compensate for ERK2 knockdown. Cell-tracing experiments revealed a convergence defect for ERK1 morphants without a severe posterior-extension defect, whereas ERK2 morphants showed a more severe reduction in anterior-posterior extension. These defects were primary changes in gastrulation cell movements and not caused by altered cell fate specification. Saturating knockdown conditions showed that the absence of FGF-mediated dual-phosphorylated ERK2 from the blastula margin blocked initiation of epiboly, actin and tubulin cytoskeleton reorganization processes and further arrested embryogenesis, whereas ERK1 knockdown had only a mild effect on epiboly progression. Together, our data define distinct roles for ERK1 and ERK2 in developmental cell migration processes during zebrafish embryogenesis.

AB - The MAPKs are key regulatory signaling molecules in many cellular processes. Here we define differential functions for ERK1 and ERK2 MAPKs in zebrafish embryogenesis. Morpholino knockdown of ERK1 and ERK2 resulted in cell migration defects during gastrulation, which could be rescued by co-injection of the corresponding mRNA. Strikingly, Erk2 mRNA cross-rescued ERK1 knockdown, but erk1 mRNA was unable to compensate for ERK2 knockdown. Cell-tracing experiments revealed a convergence defect for ERK1 morphants without a severe posterior-extension defect, whereas ERK2 morphants showed a more severe reduction in anterior-posterior extension. These defects were primary changes in gastrulation cell movements and not caused by altered cell fate specification. Saturating knockdown conditions showed that the absence of FGF-mediated dual-phosphorylated ERK2 from the blastula margin blocked initiation of epiboly, actin and tubulin cytoskeleton reorganization processes and further arrested embryogenesis, whereas ERK1 knockdown had only a mild effect on epiboly progression. Together, our data define distinct roles for ERK1 and ERK2 in developmental cell migration processes during zebrafish embryogenesis.

U2 - 10.1016/j.ydbio.2008.04.032

DO - 10.1016/j.ydbio.2008.04.032

M3 - Article

SN - 0012-1606

VL - 319

SP - 370

EP - 383

JO - Developmental Biology

JF - Developmental Biology

IS - 2

ER -

Distinct functions for ERK1 and ERK2 in cell migration processes during zebrafish gastrulation.

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