Diversity of aquatic Pseudomonas species and their activity against the fish pathogenic oomycete Saprolegnia

TY - JOUR

T1 - Diversity of aquatic Pseudomonas species and their activity against the fish pathogenic oomycete Saprolegnia

AU - Liu, Yiying

AU - Rzeszutek, E.

AU - van der Voort, M.

AU - Wu, C.H.

AU - Thoen, E.

AU - Skaar, I.

AU - Bulone, V.

AU - Dorrestein, Pieter C.

AU - Raaijmakers, J.M.

AU - De Bruijn, I.

N1 - 5912; ME; Data Archiving: Data archived at Genbank: Data have been deposited in GenBank under accession numbers KP890304-KP890314 and KT223371- T223386, respectively.

PY - 2015

Y1 - 2015

N2 - Emerging fungal and oomycete pathogens are increasingly threatening animals and plants globally. Amongst oomycetes, Saprolegnia species adversely affect wild and cultivated populations of amphibians and fish, leading to substantial reductions in biodiversity and food productivity. With the ban of several chemical control measures, new sustainable methods are needed to mitigate Saprolegnia infections in aquaculture. Here, PhyloChip-based community analyses showed that the Pseudomonadales, particularly Pseudomonas species, represent one of the largest bacterial orders associated with salmon eggs from a commercial hatchery. Among the Pseudomonas species isolated from salmon eggs, significantly more biosurfactant producers were retrieved from healthy salmon eggs than from Saprolegnia-infected eggs. Subsequent in vivo activity bioassays showed that Pseudomonas isolate H6 significantly reduced salmon egg mortality caused by Saprolegnia diclina. Live colony mass spectrometry showed that strain H6 produces a viscosin-like lipopeptide surfactant. This biosurfactant inhibited growth of Saprolegnia in vitro, but no significant protection of salmon eggs against Saprolegniosis was observed. These results indicate that live inocula of aquatic Pseudomonas strains, instead of their bioactive compound, can provide new (micro)biological and sustainable means to mitigate oomycete diseases in aquaculture.

AB - Emerging fungal and oomycete pathogens are increasingly threatening animals and plants globally. Amongst oomycetes, Saprolegnia species adversely affect wild and cultivated populations of amphibians and fish, leading to substantial reductions in biodiversity and food productivity. With the ban of several chemical control measures, new sustainable methods are needed to mitigate Saprolegnia infections in aquaculture. Here, PhyloChip-based community analyses showed that the Pseudomonadales, particularly Pseudomonas species, represent one of the largest bacterial orders associated with salmon eggs from a commercial hatchery. Among the Pseudomonas species isolated from salmon eggs, significantly more biosurfactant producers were retrieved from healthy salmon eggs than from Saprolegnia-infected eggs. Subsequent in vivo activity bioassays showed that Pseudomonas isolate H6 significantly reduced salmon egg mortality caused by Saprolegnia diclina. Live colony mass spectrometry showed that strain H6 produces a viscosin-like lipopeptide surfactant. This biosurfactant inhibited growth of Saprolegnia in vitro, but no significant protection of salmon eggs against Saprolegniosis was observed. These results indicate that live inocula of aquatic Pseudomonas strains, instead of their bioactive compound, can provide new (micro)biological and sustainable means to mitigate oomycete diseases in aquaculture.

KW - international

UR - https://www.ncbi.nlm.nih.gov/popset/925170675

UR - https://www.ncbi.nlm.nih.gov/popset/925170686

U2 - 10.1371/journal.pone.0136241

DO - 10.1371/journal.pone.0136241

M3 - Article

SN - 1932-6203

VL - 10

JO - PLoS One

JF - PLoS One

IS - 8

M1 - e0136241

ER -