Dll1+ secretory progenitor cells revert to stem cells upon crypt damage

J.H. van Es; T. Sato; M. van de Wetering; A. Lyubimova; A.N. Nee; A. Gregorieff; N. Sasaki; L. Zeinstra; M. van den Born; J. Korving; A.C. Martens; N. Barker; A. van Oudenaarden; H. Clevers

doi:10.1038/ncb2581

Dll1+ secretory progenitor cells revert to stem cells upon crypt damage

J.H. van Es, T. Sato, M. van de Wetering, A. Lyubimova, A.N. Nee, A. Gregorieff, N. Sasaki, L. Zeinstra, M. van den Born, J. Korving, A.C. Martens, N. Barker, A. van Oudenaarden, H. Clevers

Hubrecht Institute

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

596 Citaten (Scopus)

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Lgr5+ intestinal stem cells generate enterocytes and secretory cells. Secretory lineage commitment requires Notch silencing. The Notch ligand Dll1 is expressed by a subset of immediate stem cell daughters. Lineage tracing in Dll1(GFP-ires-CreERT2) knock-in mice reveals that single Dll1(high) cells generate small, short-lived clones containing all four secretory cell types. Lineage specification thus occurs in immediate stem cell daughters through Notch lateral inhibition. Cultured Dll1(high) cells form long-lived organoids (mini-guts) on brief Wnt3A exposure. When Dll1(high) cells are genetically marked before tissue damage, stem cell tracing events occur. Thus, secretory progenitors exhibit plasticity by regaining stemness on damage.

Originele taal-2	Engels
Pagina's (van-tot)	1099-1104
Tijdschrift	Nature Cell Biology
Volume	14
Nummer van het tijdschrift	10
DOI's	https://doi.org/10.1038/ncb2581
Status	Gepubliceerd - 2012

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@article{26d0e75f6d994a0c925be3e19c32ed51,

title = "Dll1+ secretory progenitor cells revert to stem cells upon crypt damage",

abstract = "Lgr5+ intestinal stem cells generate enterocytes and secretory cells. Secretory lineage commitment requires Notch silencing. The Notch ligand Dll1 is expressed by a subset of immediate stem cell daughters. Lineage tracing in Dll1(GFP-ires-CreERT2) knock-in mice reveals that single Dll1(high) cells generate small, short-lived clones containing all four secretory cell types. Lineage specification thus occurs in immediate stem cell daughters through Notch lateral inhibition. Cultured Dll1(high) cells form long-lived organoids (mini-guts) on brief Wnt3A exposure. When Dll1(high) cells are genetically marked before tissue damage, stem cell tracing events occur. Thus, secretory progenitors exhibit plasticity by regaining stemness on damage.",

author = "{van Es}, J.H. and T. Sato and {van de Wetering}, M. and A. Lyubimova and A.N. Nee and A. Gregorieff and N. Sasaki and L. Zeinstra and {van den Born}, M. and J. Korving and A.C. Martens and N. Barker and {van Oudenaarden}, A. and H. Clevers",

note = "Reporting year: 2012",

year = "2012",

doi = "10.1038/ncb2581",

language = "English",

volume = "14",

pages = "1099--1104",

journal = "Nature Cell Biology",

issn = "1465-7392",

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T1 - Dll1+ secretory progenitor cells revert to stem cells upon crypt damage

AU - van Es, J.H.

AU - Sato, T.

AU - van de Wetering, M.

AU - Lyubimova, A.

AU - Nee, A.N.

AU - Gregorieff, A.

AU - Sasaki, N.

AU - Zeinstra, L.

AU - van den Born, M.

AU - Korving, J.

AU - Martens, A.C.

AU - Barker, N.

AU - van Oudenaarden, A.

AU - Clevers, H.

N1 - Reporting year: 2012

PY - 2012

Y1 - 2012

N2 - Lgr5+ intestinal stem cells generate enterocytes and secretory cells. Secretory lineage commitment requires Notch silencing. The Notch ligand Dll1 is expressed by a subset of immediate stem cell daughters. Lineage tracing in Dll1(GFP-ires-CreERT2) knock-in mice reveals that single Dll1(high) cells generate small, short-lived clones containing all four secretory cell types. Lineage specification thus occurs in immediate stem cell daughters through Notch lateral inhibition. Cultured Dll1(high) cells form long-lived organoids (mini-guts) on brief Wnt3A exposure. When Dll1(high) cells are genetically marked before tissue damage, stem cell tracing events occur. Thus, secretory progenitors exhibit plasticity by regaining stemness on damage.

AB - Lgr5+ intestinal stem cells generate enterocytes and secretory cells. Secretory lineage commitment requires Notch silencing. The Notch ligand Dll1 is expressed by a subset of immediate stem cell daughters. Lineage tracing in Dll1(GFP-ires-CreERT2) knock-in mice reveals that single Dll1(high) cells generate small, short-lived clones containing all four secretory cell types. Lineage specification thus occurs in immediate stem cell daughters through Notch lateral inhibition. Cultured Dll1(high) cells form long-lived organoids (mini-guts) on brief Wnt3A exposure. When Dll1(high) cells are genetically marked before tissue damage, stem cell tracing events occur. Thus, secretory progenitors exhibit plasticity by regaining stemness on damage.

U2 - 10.1038/ncb2581

DO - 10.1038/ncb2581

M3 - Article

SN - 1465-7392

VL - 14

SP - 1099

EP - 1104

JO - Nature Cell Biology

JF - Nature Cell Biology

IS - 10

ER -

Dll1+ secretory progenitor cells revert to stem cells upon crypt damage

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