DNA barcoding of clinically relevant Cunninghamella species

TY - JOUR

T1 - DNA barcoding of clinically relevant Cunninghamella species

AU - Yu, Jin

AU - Walther, G

AU - Van Diepeningen, A D

AU - Gerrits Van Den Ende, A H G

AU - Li, Ruo-Yu

AU - Moussa, T A A

AU - Almaghrabi, O A

AU - De Hoog, G S

N1 - © The Author 2014. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology. All rights reserved. For permissions, please e-mail: [email protected].

PY - 2015/2/1

Y1 - 2015/2/1

N2 - Mucormycosis caused, in part, by representatives of the genus Cunninghamella is a severe infection with high mortality in patients with impaired immunity. Several species have been described in the literature as etiologic agents. A DNA barcoding study using ITS rDNA and tef-1α provided concordance of molecular data with conventional characters. The currently accepted Cunninghamella species were well supported in phylogenetic trees of both markers except for C. septata with ITS that clustered in the C. echinulata clade. Sequence variability was distinctly higher for the ITS than for tef-1α. Intraspecific ITS variability of some of the species exceeded that between some closely related species, but the marker remained applicable for species identification. The most variable species for both markers was C. echinulata. Cunninghamella bertholletiae is the main pathogenic species; infections by C. blakesleeana, C. echinulata, and C. elegans are highly exceptional.

AB - Mucormycosis caused, in part, by representatives of the genus Cunninghamella is a severe infection with high mortality in patients with impaired immunity. Several species have been described in the literature as etiologic agents. A DNA barcoding study using ITS rDNA and tef-1α provided concordance of molecular data with conventional characters. The currently accepted Cunninghamella species were well supported in phylogenetic trees of both markers except for C. septata with ITS that clustered in the C. echinulata clade. Sequence variability was distinctly higher for the ITS than for tef-1α. Intraspecific ITS variability of some of the species exceeded that between some closely related species, but the marker remained applicable for species identification. The most variable species for both markers was C. echinulata. Cunninghamella bertholletiae is the main pathogenic species; infections by C. blakesleeana, C. echinulata, and C. elegans are highly exceptional.

U2 - 10.1093/mmy/myu079

DO - 10.1093/mmy/myu079

M3 - Article

C2 - 25431472

SN - 1369-3786

VL - 53

SP - 99

EP - 106

JO - Medical Mycology

JF - Medical Mycology

IS - 2

ER -