DNA barcoding of clinically relevant Cunninghamella species

Jin Yu, G Walther, A D Van Diepeningen, A H G Gerrits Van Den Ende, Ruo-Yu Li, T A A Moussa, O A Almaghrabi, G S De Hoog

    Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgaveArtikelWetenschappelijkpeer review


    Mucormycosis caused, in part, by representatives of the genus Cunninghamella is a severe infection with high mortality in patients with impaired immunity. Several species have been described in the literature as etiologic agents. A DNA barcoding study using ITS rDNA and tef-1α provided concordance of molecular data with conventional characters. The currently accepted Cunninghamella species were well supported in phylogenetic trees of both markers except for C. septata with ITS that clustered in the C. echinulata clade. Sequence variability was distinctly higher for the ITS than for tef-1α. Intraspecific ITS variability of some of the species exceeded that between some closely related species, but the marker remained applicable for species identification. The most variable species for both markers was C. echinulata. Cunninghamella bertholletiae is the main pathogenic species; infections by C. blakesleeana, C. echinulata, and C. elegans are highly exceptional.

    Originele taal-2Engels
    Pagina's (van-tot)99-106
    Aantal pagina's8
    TijdschriftMedical Mycology
    Nummer van het tijdschrift2
    StatusGepubliceerd - 01 feb. 2015


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