TY - JOUR
T1 - Does the circadian clock make RPE-mediated ion transport "tick" via SLC12A2 (NKCC1)?
AU - Milićević, Nemanja
AU - Duursma, Angelica
AU - Ten Asbroek, Anneloor L M A
AU - Felder-Schmittbuhl, Marie-Paule
AU - Bergen, Arthur A
PY - 2019
Y1 - 2019
N2 - The presence of a circadian clock in the retinal pigment epithelium (RPE) was discovered recently. However, little is known about mechanisms or processes regulated by the RPE clock. We cultured ARPE-19 monolayers in a transwell culture system, and we found rhythmic mRNA expression of the sodium-potassium-chloride co-transporter SLC12A2. We localized the corresponding protein product, NKCC1, on the apical membrane of ARPE-19 cells. We found that concentrations of sodium, potassium, and chloride oscillated in apical supernatants. The ion concentration gradients between supernatants strongly correlated with SLC12A2 mRNA expression. Our results suggest that the circadian clock regulates ion transport by the RPE via NKCC1 expression.
AB - The presence of a circadian clock in the retinal pigment epithelium (RPE) was discovered recently. However, little is known about mechanisms or processes regulated by the RPE clock. We cultured ARPE-19 monolayers in a transwell culture system, and we found rhythmic mRNA expression of the sodium-potassium-chloride co-transporter SLC12A2. We localized the corresponding protein product, NKCC1, on the apical membrane of ARPE-19 cells. We found that concentrations of sodium, potassium, and chloride oscillated in apical supernatants. The ion concentration gradients between supernatants strongly correlated with SLC12A2 mRNA expression. Our results suggest that the circadian clock regulates ion transport by the RPE via NKCC1 expression.
U2 - 10.1080/07420528.2019.1653317
DO - 10.1080/07420528.2019.1653317
M3 - Article
C2 - 31441327
SN - 0742-0528
VL - 36
SP - 1592
EP - 1598
JO - Chronobiology International
JF - Chronobiology International
IS - 11
ER -