Dominant hyphae-associated bacteria of Fusarium oxysporum f. sp. cucumerinum in different cropping systems and insight into their functions

TY - JOUR

T1 - Dominant hyphae-associated bacteria of Fusarium oxysporum f. sp. cucumerinum in different cropping systems and insight into their functions

AU - Sun, Rui Liang

AU - Jing, Yu Ling

AU - de Boer, Wietse

AU - Guo, Rong-Jun

AU - Li, Shi Dong

N1 - 7229, ME; Data archiving: data archived at NCBI (no NIOO data)

PY - 2021

Y1 - 2021

N2 - Serious cucumber fusarium wilt (CFW) caused by the pathogenic fungus Fusarium oxysporum f. sp. cucumerinum (Foc) frequently occurs during continuous cultivation. The soil bacteria colonized in Foc hyphosphere are supposed to be related to Foc performance and cucumber health. In the present study, culture-independent approach was used to examine the Foc hyphae-associated bacterial communities in different cucumber cropping systems. It was found that the assembly of Foc hyphae-associated bacterial communities was mainly affected by the continuous cucumber practice followed by the pathogen colonization. Proteobacteria, Actinobacteria and Bacteroidetes accounted for 89–93% of the Foc hyphae-associated bacteria, and their abundance changed along with cucumber growth and Foc presence. The abundance of dominant Achromobacter was driven by cucumber cultivation, while the abundance of Rhizobium increased with continuous cucumber cultivation but decreased by Foc spiking. Bioassay tests of cultivable bacterial strains showed that the proportion of Rhizobium and Achromobacter played a key role in the occurrence of CFW, i.e. decreased Rhizobium and increased Achromobacter in the bacterial mixtures led to the decline of Foc suppression. This study indicates the relationship of Foc hyphae-associated bacterial community and cropping systems, and reveals the roles of some specific assembly of dominant bacteria colonizing Foc hyphae in CFW suppression.

AB - Serious cucumber fusarium wilt (CFW) caused by the pathogenic fungus Fusarium oxysporum f. sp. cucumerinum (Foc) frequently occurs during continuous cultivation. The soil bacteria colonized in Foc hyphosphere are supposed to be related to Foc performance and cucumber health. In the present study, culture-independent approach was used to examine the Foc hyphae-associated bacterial communities in different cucumber cropping systems. It was found that the assembly of Foc hyphae-associated bacterial communities was mainly affected by the continuous cucumber practice followed by the pathogen colonization. Proteobacteria, Actinobacteria and Bacteroidetes accounted for 89–93% of the Foc hyphae-associated bacteria, and their abundance changed along with cucumber growth and Foc presence. The abundance of dominant Achromobacter was driven by cucumber cultivation, while the abundance of Rhizobium increased with continuous cucumber cultivation but decreased by Foc spiking. Bioassay tests of cultivable bacterial strains showed that the proportion of Rhizobium and Achromobacter played a key role in the occurrence of CFW, i.e. decreased Rhizobium and increased Achromobacter in the bacterial mixtures led to the decline of Foc suppression. This study indicates the relationship of Foc hyphae-associated bacterial community and cropping systems, and reveals the roles of some specific assembly of dominant bacteria colonizing Foc hyphae in CFW suppression.

KW - Continuous cucumber cropping

KW - Cucumber fusarium wilt (CFW)

KW - Disease suppression

KW - Fusarium oxysporum f. sp. cucumerinum (Foc)

KW - Hyphae-associated bacteria

KW - international

KW - Plan_S-Compliant_NO

U2 - 10.1016/j.apsoil.2021.103977

DO - 10.1016/j.apsoil.2021.103977

M3 - Article

AN - SCOPUS:85102874325

SN - 0929-1393

VL - 165

JO - Applied Soil Ecology

JF - Applied Soil Ecology

M1 - 103977

ER -