A low dose flocculant (FeCl3), combined with lanthanum modified bentonite (LMB) as phosphate-binding agent, has been applied for eutrophication management in Lake De Kuil (The Netherlands). After the treatment, the state of the lake shifted from hypertrophic to mesotrophic. Although macroinvertebrate fauna is important for lake ecosystems, the knowledge of its response to this lake restoration method is fragmented and scarce. Because insight in the macroinvertebrate fauna response is important to assess future applications, pre and post application macroinvertebrate assemblages were identified in Lake De Kuil. The research was accompanied by a microcosm experiment in which the effects of LMB, FeCl3 and LMB + FeCl3 were studied on macroinvertebrate communities. Results show the reduction of macroinvertebrate numbers and taxa during the first month following the application. The number of Gastropoda was strikingly reduced one month after the application. One year after the application, the macroinvertebrate numbers and taxa exceeded the pre-application situation and Gastropoda and Oligochaeta prospered. The effects one month after the treatment are most likely due to the combination of physical impacts of the use of bentonite and chemical impacts of the use of FeCl3, while effects after one year are likely attributed to the shift in trophic state of the lake.