Enhanced surface colonisation and competition during bacterial adaptation to a fungus

Anne Richter, Felix Blei, Guohai Hu, Jan W. Schwitalla, Carlos N. Lozano-Andrade, Jiyu Xie, Scott A. Jarmusch, Mario Wibowo, Bodil Kjeldgaard, Surabhi Surabhi, Xinming Xu, Theresa Jautzus, Christopher B. W. Phippen, Olaf Tyc, Mark Arentshorst, Yue Wang, Paolina Garbeva, Thomas Ostenfeld Larsen, Arthur F. J. Ram, Cees A. M. van den HondelGergely Maróti, Ákos T. Kovács* (Co-auteur)

*Bijbehorende auteur voor dit werk

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgaveArtikelWetenschappelijkpeer review

3 Citaten (Scopus)

Samenvatting

Bacterial-fungal interactions influence microbial community performance of most ecosystems and elicit specific microbial behaviours, including stimulating specialised metabolite production. Here, we use a co-culture experimental evolution approach to investigate bacterial adaptation to the presence of a fungus, using a simple model of bacterial-fungal interactions encompassing the bacterium Bacillus subtilis and the fungus Aspergillus niger. We find in one evolving population that B. subtilis was selected for enhanced production of the lipopeptide surfactin and accelerated surface spreading ability, leading to inhibition of fungal expansion and acidification of the environment. These phenotypes were explained by specific mutations in the DegS-DegU two-component system. In the presence of surfactin, fungal hyphae exhibited bulging cells with delocalised secretory vesicles possibly provoking an RlmA-dependent cell wall stress. Thus, our results indicate that the presence of the fungus selects for increased surfactin production, which inhibits fungal growth and facilitates the competitive success of the bacterium.
Originele taal-2Engels
Artikelnummer4486
TijdschriftNature Communications
Volume15
DOI's
StatusGepubliceerd - 27 mei 2024

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