Enterovirus 71 infection of human airway organoids reveals VP1-145 as a viral infectivity determinant

Sabine M G van der Sanden, Norman Sachs, Sylvie M Koekkoek, Gerrit Koen, Dasja Pajkrt, Hans Clevers, Katja C Wolthers

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgaveArtikelWetenschappelijkpeer review

Samenvatting

Human enteroviruses frequently cause severe diseases in children. Human enteroviruses are transmitted via the fecal-oral route and respiratory droplets, and primary replication occurs in the gastro-intestinal and respiratory tracts; however, how enteroviruses infect these sites is largely unknown. Human intestinal organoids have recently proven to be valuable tools for studying enterovirus-host interactions in the intestinal tract. In this study, we demonstrated the susceptibility of a newly developed human airway organoid model for enterovirus 71 (EV71) infection. We showed for the first time in a human physiological model that EV71 replication kinetics are strain-dependent. A glutamine at position 145 of the VP1 capsid protein was identified as a key determinant of infectivity, and residues VP1-98K and VP1-104D were identified as potential infectivity markers. The results from this study provide new insights into EV71 infectivity in the human airway epithelia and demonstrate the value of organoid technology for virus research.

Originele taal-2Engels
Pagina's (van-tot)84
TijdschriftEmerging Microbes & Infections
Volume7
Nummer van het tijdschrift1
DOI's
StatusGepubliceerd - 09 mei 2018

Vingerafdruk

Duik in de onderzoeksthema's van 'Enterovirus 71 infection of human airway organoids reveals VP1-145 as a viral infectivity determinant'. Samen vormen ze een unieke vingerafdruk.

Citeer dit