ENU mutagenesis to generate genetically modified rat models

TY - JOUR

T1 - ENU mutagenesis to generate genetically modified rat models

AU - van Boxtel, R.

AU - Gould, M.

AU - Cuppen, E.

AU - Smits, B.M.

N1 - Reporting year: 2010

PY - 2010

Y1 - 2010

N2 - The rat is one of the most preferred model organisms in biomedical research and has been extremely useful for linking physiology and pathology to the genome. However, approaches to genetically modify specific genes in the rat germ line remain relatively scarce. To date, the most efficient approach for generating genetically modified rats has been the target-selected N-ethyl-N-nitrosourea (ENU) mutagenesis-based technology. Here, we describe the detailed protocols for ENU mutagenesis and mutant retrieval in the rat model organism.

AB - The rat is one of the most preferred model organisms in biomedical research and has been extremely useful for linking physiology and pathology to the genome. However, approaches to genetically modify specific genes in the rat germ line remain relatively scarce. To date, the most efficient approach for generating genetically modified rats has been the target-selected N-ethyl-N-nitrosourea (ENU) mutagenesis-based technology. Here, we describe the detailed protocols for ENU mutagenesis and mutant retrieval in the rat model organism.

U2 - 10.1007/978-1-60327-389-3_11

DO - 10.1007/978-1-60327-389-3_11

M3 - Article

SN - 1064-3745

VL - 597

SP - 151

EP - 167

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -