Environmental and genealogical effects on DNA methylation in a widespread apomictic dandelion lineage

Verónica Noé Ibañez, Morgane van Antro, Cristian Peña‐Ponton, Slavica Milanovic‐Ivanovic, Cornelis A. M. Wagemaker, Fleur Gawehns, Koen J. F. Verhoeven* (Co-auteur)

*Bijbehorende auteur voor dit werk

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgaveArtikelWetenschappelijkpeer review

2 Citaten (Scopus)
41 Downloads (Pure)

Samenvatting

DNA methylation in plant genomes occurs in different sequences and genomic contexts that have very different properties. DNA methylation that occurs in CG (mCG) sequence context shows transgenerational stability and high epimutation rate, and can thus provide genealogical information at short time scales. However, due to meta-stability and because mCG variants may arise due to other factors than epimutation, such as environmental stress exposure, it is not clear how well mCG captures genealogical information at micro-evolutionary time scales. Here, we analysed DNA methylation variation between accessions from a geographically widespread, apomictic common dandelion (Taraxacum officinale) lineage when grown experimentally under different light conditions. Using a reduced-representation bisulphite sequencing approach, we show that the light treatment induced differentially methylated cytosines (DMCs) in all sequence contexts, with a bias towards transposable elements. Accession differences were associated mainly with DMCs in CG context. Hierarchical clustering of samples based on total mCG profiles revealed a perfect clustering of samples by accession identity, irrespective of light conditions. Using microsatellite information as a benchmark of genetic divergence within the clonal lineage, we show that genetic divergence between accessions correlates strongly with overall mCG profiles. However, our results suggest that environmental effects that do occur in CG context may produce a heritable signal that partly dilutes the genealogical signal. Our study shows that methylation information in plants can be used to reconstruct micro-evolutionary genealogy, providing a useful tool in systems that lack genetic variation such as clonal and vegetatively propagated plants.
Originele taal-2Engels
Pagina's (van-tot)663-674
Aantal pagina's12
TijdschriftJournal of Evolutionary Biology
Volume36
Nummer van het tijdschrift4
DOI's
StatusE-pub ahead of print - 22 feb. 2023

Vingerafdruk

Duik in de onderzoeksthema's van 'Environmental and genealogical effects on DNA methylation in a widespread apomictic dandelion lineage'. Samen vormen ze een unieke vingerafdruk.

Citeer dit