Epidemiology of yeast species causing bloodstream infection in Tehran, Iran (2015-2017); superiority of 21-plex PCR over the Vitek 2 system for yeast identification

Mohammad Kord; Mohammadreza Salehi; Sadegh Khodavaisy; Seyed Jamal Hashemi; Roshanak Daie Ghazvini; Sassan Rezaei; Ayda Maleki; Ahmad Elmimoghaddam; Neda Alijani; Alireza Abdollahi; Mahsa Doomanlou; Kazem Ahmadikia; Niloofar Rashidi; Weihua Pan; Teun Boekhout; Amir Arastehfar

doi:10.1099/jmm.0.001189

Epidemiology of yeast species causing bloodstream infection in Tehran, Iran (2015-2017); superiority of 21-plex PCR over the Vitek 2 system for yeast identification

Mohammad Kord, Mohammadreza Salehi, Sadegh Khodavaisy, Seyed Jamal Hashemi, Roshanak Daie Ghazvini, Sassan Rezaei, Ayda Maleki, Ahmad Elmimoghaddam, Neda Alijani, Alireza Abdollahi, Mahsa Doomanlou, Kazem Ahmadikia, Niloofar Rashidi, Weihua Pan, Teun Boekhout, Amir Arastehfar

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

26 Citaten (Scopus)

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Introduction. Given the limited number of candidaemia studies in Iran, the profile of yeast species causing bloodstream infections (BSIs), especially in adults, remains limited. Although biochemical assays are widely used in developing countries, they produce erroneous results, especially for rare yeast species.Aim. We aimed to assess the profile of yeast species causing BSIs and to compare the accuracy of the Vitek 2 system and 21-plex PCR.Methodology. Yeast blood isolates were retrospectively collected from patients recruited from two tertiary care training hospitals in Tehran from 2015 to 2017. Relevant clinical data were mined. Identification was performed by automated Vitek 2, 21-plex PCR and sequencing of the internal transcribed spacer region (ITS1-5.8S-ITS2).Results. In total, 137 yeast isolates were recovered from 107 patients. The overall all-cause 30-day mortality rate was 47.7 %. Fluconazole was the most widely used systemic antifungal. Candida albicans (58/137, 42.3 %), Candida glabrata (30/137, 21.9 %), Candida parapsilosis sensu stricto (23/137, 16.8 %), Candida tropicalis (10/137, 7.3 %) and Pichia kudriavzevii (Candida krusei) (4/137, 2.9 %) constituted almost 90 % of the isolates and 10 % of the species detected were rare yeast species (12/137; 8.7 %). The 21-plex PCR method correctly identified 97.1 % of the isolates, a higher percentage than the Vitek 2 showed (87.6 %).Conclusion.C. albicans was the main cause of yeast-derived fungaemia in this study. Future prospective studies are warranted to closely monitor the epidemiological landscape of yeast species causing BSIs in Iran. The superiority of 21-plex PCR over automated Vitek 2 indicates its potential clinical utility as an alternative identification tool use in developing countries.

Originele taal-2	Engels
Pagina's (van-tot)	712-720
Aantal pagina's	9
Tijdschrift	Journal of Medical Microbiology
Volume	69
Nummer van het tijdschrift	5
DOI's	https://doi.org/10.1099/jmm.0.001189
Status	Gepubliceerd - mei 2020

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10.1099/jmm.0.001189

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Kord, M., Salehi, M., Khodavaisy, S., Hashemi, S. J., Daie Ghazvini, R., Rezaei, S., Maleki, A., Elmimoghaddam, A., Alijani, N., Abdollahi, A., Doomanlou, M., Ahmadikia, K., Rashidi, N., Pan, W., Boekhout, T., & Arastehfar, A. (2020). Epidemiology of yeast species causing bloodstream infection in Tehran, Iran (2015-2017); superiority of 21-plex PCR over the Vitek 2 system for yeast identification. Journal of Medical Microbiology, 69(5), 712-720. https://doi.org/10.1099/jmm.0.001189

@article{7271ce71e57346a5b012ba73c3935c81,

title = "Epidemiology of yeast species causing bloodstream infection in Tehran, Iran (2015-2017); superiority of 21-plex PCR over the Vitek 2 system for yeast identification",

abstract = "Introduction. Given the limited number of candidaemia studies in Iran, the profile of yeast species causing bloodstream infections (BSIs), especially in adults, remains limited. Although biochemical assays are widely used in developing countries, they produce erroneous results, especially for rare yeast species.Aim. We aimed to assess the profile of yeast species causing BSIs and to compare the accuracy of the Vitek 2 system and 21-plex PCR.Methodology. Yeast blood isolates were retrospectively collected from patients recruited from two tertiary care training hospitals in Tehran from 2015 to 2017. Relevant clinical data were mined. Identification was performed by automated Vitek 2, 21-plex PCR and sequencing of the internal transcribed spacer region (ITS1-5.8S-ITS2).Results. In total, 137 yeast isolates were recovered from 107 patients. The overall all-cause 30-day mortality rate was 47.7 %. Fluconazole was the most widely used systemic antifungal. Candida albicans (58/137, 42.3 %), Candida glabrata (30/137, 21.9 %), Candida parapsilosis sensu stricto (23/137, 16.8 %), Candida tropicalis (10/137, 7.3 %) and Pichia kudriavzevii (Candida krusei) (4/137, 2.9 %) constituted almost 90 % of the isolates and 10 % of the species detected were rare yeast species (12/137; 8.7 %). The 21-plex PCR method correctly identified 97.1 % of the isolates, a higher percentage than the Vitek 2 showed (87.6 %).Conclusion.C. albicans was the main cause of yeast-derived fungaemia in this study. Future prospective studies are warranted to closely monitor the epidemiological landscape of yeast species causing BSIs in Iran. The superiority of 21-plex PCR over automated Vitek 2 indicates its potential clinical utility as an alternative identification tool use in developing countries.",

keywords = "Aged, Aged, 80 and over, DNA, Intergenic, Female, Fungemia/diagnosis, History, 21st Century, Humans, Iran/epidemiology, Male, Middle Aged, Multiplex Polymerase Chain Reaction/methods, Sequence Analysis, DNA/methods, Yeasts/classification",

author = "Mohammad Kord and Mohammadreza Salehi and Sadegh Khodavaisy and Hashemi, {Seyed Jamal} and {Daie Ghazvini}, Roshanak and Sassan Rezaei and Ayda Maleki and Ahmad Elmimoghaddam and Neda Alijani and Alireza Abdollahi and Mahsa Doomanlou and Kazem Ahmadikia and Niloofar Rashidi and Weihua Pan and Teun Boekhout and Amir Arastehfar",

year = "2020",

month = may,

doi = "10.1099/jmm.0.001189",

language = "English",

volume = "69",

pages = "712--720",

journal = "Journal of Medical Microbiology",

issn = "0022-2615",

publisher = "Society for General Microbiology",

number = "5",

}

Kord, M, Salehi, M, Khodavaisy, S, Hashemi, SJ, Daie Ghazvini, R, Rezaei, S, Maleki, A, Elmimoghaddam, A, Alijani, N, Abdollahi, A, Doomanlou, M, Ahmadikia, K, Rashidi, N, Pan, W, Boekhout, T & Arastehfar, A 2020, 'Epidemiology of yeast species causing bloodstream infection in Tehran, Iran (2015-2017); superiority of 21-plex PCR over the Vitek 2 system for yeast identification', Journal of Medical Microbiology, vol. 69, nr. 5, blz. 712-720. https://doi.org/10.1099/jmm.0.001189

Epidemiology of yeast species causing bloodstream infection in Tehran, Iran (2015-2017); superiority of 21-plex PCR over the Vitek 2 system for yeast identification. / Kord, Mohammad; Salehi, Mohammadreza; Khodavaisy, Sadegh et al.
In: Journal of Medical Microbiology, Vol. 69, Nr. 5, 05.2020, blz. 712-720.

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

TY - JOUR

T1 - Epidemiology of yeast species causing bloodstream infection in Tehran, Iran (2015-2017); superiority of 21-plex PCR over the Vitek 2 system for yeast identification

AU - Kord, Mohammad

AU - Salehi, Mohammadreza

AU - Khodavaisy, Sadegh

AU - Hashemi, Seyed Jamal

AU - Daie Ghazvini, Roshanak

AU - Rezaei, Sassan

AU - Maleki, Ayda

AU - Elmimoghaddam, Ahmad

AU - Alijani, Neda

AU - Abdollahi, Alireza

AU - Doomanlou, Mahsa

AU - Ahmadikia, Kazem

AU - Rashidi, Niloofar

AU - Pan, Weihua

AU - Boekhout, Teun

AU - Arastehfar, Amir

PY - 2020/5

Y1 - 2020/5

N2 - Introduction. Given the limited number of candidaemia studies in Iran, the profile of yeast species causing bloodstream infections (BSIs), especially in adults, remains limited. Although biochemical assays are widely used in developing countries, they produce erroneous results, especially for rare yeast species.Aim. We aimed to assess the profile of yeast species causing BSIs and to compare the accuracy of the Vitek 2 system and 21-plex PCR.Methodology. Yeast blood isolates were retrospectively collected from patients recruited from two tertiary care training hospitals in Tehran from 2015 to 2017. Relevant clinical data were mined. Identification was performed by automated Vitek 2, 21-plex PCR and sequencing of the internal transcribed spacer region (ITS1-5.8S-ITS2).Results. In total, 137 yeast isolates were recovered from 107 patients. The overall all-cause 30-day mortality rate was 47.7 %. Fluconazole was the most widely used systemic antifungal. Candida albicans (58/137, 42.3 %), Candida glabrata (30/137, 21.9 %), Candida parapsilosis sensu stricto (23/137, 16.8 %), Candida tropicalis (10/137, 7.3 %) and Pichia kudriavzevii (Candida krusei) (4/137, 2.9 %) constituted almost 90 % of the isolates and 10 % of the species detected were rare yeast species (12/137; 8.7 %). The 21-plex PCR method correctly identified 97.1 % of the isolates, a higher percentage than the Vitek 2 showed (87.6 %).Conclusion.C. albicans was the main cause of yeast-derived fungaemia in this study. Future prospective studies are warranted to closely monitor the epidemiological landscape of yeast species causing BSIs in Iran. The superiority of 21-plex PCR over automated Vitek 2 indicates its potential clinical utility as an alternative identification tool use in developing countries.

AB - Introduction. Given the limited number of candidaemia studies in Iran, the profile of yeast species causing bloodstream infections (BSIs), especially in adults, remains limited. Although biochemical assays are widely used in developing countries, they produce erroneous results, especially for rare yeast species.Aim. We aimed to assess the profile of yeast species causing BSIs and to compare the accuracy of the Vitek 2 system and 21-plex PCR.Methodology. Yeast blood isolates were retrospectively collected from patients recruited from two tertiary care training hospitals in Tehran from 2015 to 2017. Relevant clinical data were mined. Identification was performed by automated Vitek 2, 21-plex PCR and sequencing of the internal transcribed spacer region (ITS1-5.8S-ITS2).Results. In total, 137 yeast isolates were recovered from 107 patients. The overall all-cause 30-day mortality rate was 47.7 %. Fluconazole was the most widely used systemic antifungal. Candida albicans (58/137, 42.3 %), Candida glabrata (30/137, 21.9 %), Candida parapsilosis sensu stricto (23/137, 16.8 %), Candida tropicalis (10/137, 7.3 %) and Pichia kudriavzevii (Candida krusei) (4/137, 2.9 %) constituted almost 90 % of the isolates and 10 % of the species detected were rare yeast species (12/137; 8.7 %). The 21-plex PCR method correctly identified 97.1 % of the isolates, a higher percentage than the Vitek 2 showed (87.6 %).Conclusion.C. albicans was the main cause of yeast-derived fungaemia in this study. Future prospective studies are warranted to closely monitor the epidemiological landscape of yeast species causing BSIs in Iran. The superiority of 21-plex PCR over automated Vitek 2 indicates its potential clinical utility as an alternative identification tool use in developing countries.

KW - Aged

KW - Aged, 80 and over

KW - DNA, Intergenic

KW - Female

KW - Fungemia/diagnosis

KW - History, 21st Century

KW - Humans

KW - Iran/epidemiology

KW - Male

KW - Middle Aged

KW - Multiplex Polymerase Chain Reaction/methods

KW - Sequence Analysis, DNA/methods

KW - Yeasts/classification

U2 - 10.1099/jmm.0.001189

DO - 10.1099/jmm.0.001189

M3 - Article

C2 - 32368996

SN - 0022-2615

VL - 69

SP - 712

EP - 720

JO - Journal of Medical Microbiology

JF - Journal of Medical Microbiology

IS - 5

ER -

Epidemiology of yeast species causing bloodstream infection in Tehran, Iran (2015-2017); superiority of 21-plex PCR over the Vitek 2 system for yeast identification

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