Projecten per jaar
Samenvatting
We describe epiGBS, a reduced representation bisulfite sequencing method for cost-effective exploration and comparative analysis of DNA methylation and genetic variation in hundreds of samples de novo. This method uses genotyping by sequencing of bisulfite-converted DNA followed by reliable de novo reference construction, mapping, variant calling, and distinction of single-nucleotide polymorphisms (SNPs) versus methylation variation (software is available at https://github.com/thomasvangurp/epiGBS). The output can be loaded directly into a genome browser for visualization and into RnBeads for analysis of differential methylation.
Originele taal-2 | Engels |
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Pagina's (van-tot) | 322-324 |
Tijdschrift | Nature Methods |
Volume | 13 |
Nummer van het tijdschrift | 4 |
DOI's | |
Status | Gepubliceerd - 2016 |
Vingerafdruk
Duik in de onderzoeksthema's van 'epiGBS: reference-free reduced representation bisulfite sequencing: brief communication'. Samen vormen ze een unieke vingerafdruk.Projecten
- 1 Afgelopen
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NWO-VIDI: Causes and consequences of heritable epigenetic variation in asexual plants
Verhoeven, K., Brinkman, P., Ferreira de Carvalho, J. & Oplaat, C.
01/02/2011 → 11/07/2016
Project: Onderzoek
Datasets
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Several species were included in this experiment:Allium porrum:4Arabidopsis thaliana:4Homo sapiens:1Fallopia japonica:4Daucus carrota:4Daphnia magna:6Scabiosa columbaria:29Mimulus guttatus:7Taraxacum officinale:35Phage lambda:1 epigenomics
Van Gurp, T. P. (Maker), Wagemaker, N. C. (Maker), Wouters, B. (Maker), Vergeer, P. (Maker), Ouborg, J. N. (Maker) & Verhoeven, K. (Maker), NCBI, 23 jun. 2015
https://www.ncbi.nlm.nih.gov/bioproject/PRJNA287755/
Dataset