epiGBS: reference-free reduced representation bisulfite sequencing: brief communication

Thomas P van Gurp, Niels CAM Wagemaker, Björn Wouters, Philippine Vergeer, Joop NJ Ouborg, Koen JF Verhoeven

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgaveArtikelWetenschappelijkpeer review

88 Citaten (Scopus)
17 Downloads (Pure)

Samenvatting

We describe epiGBS, a reduced representation bisulfite sequencing method for cost-effective exploration and comparative analysis of DNA methylation and genetic variation in hundreds of samples de novo. This method uses genotyping by sequencing of bisulfite-converted DNA followed by reliable de novo reference construction, mapping, variant calling, and distinction of single-nucleotide polymorphisms (SNPs) versus methylation variation (software is available at https://github.com/thomasvangurp/epiGBS). The output can be loaded directly into a genome browser for visualization and into RnBeads for analysis of differential methylation.

Originele taal-2Engels
Pagina's (van-tot)322-324
TijdschriftNature Methods
Volume13
Nummer van het tijdschrift4
DOI's
StatusGepubliceerd - 2016

Research theme

  • Ecological epigenetics

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