@techreport{1c165a78a92a483c9d1ef9e47a8e037a,
title = "epiGBS2: an improved protocol and automated snakemake workflow for highly multiplexed reduced representation bisulfite sequencing",
abstract = "epiGBS is an existing reduced representation bisulfite sequencing method to determine cytosine methylation and genetic polymorphisms de novo. Here, we present epiGBS2, an improved epiGBS laboratory protocol and user-friendly bioinformatics pipeline for a wide range of species with or without reference genome. epiGBS2 decreases costs and time investment and increases user-friendliness and reproducibility. The library protocol was adjusted to allow for a flexible choice of restriction enzymes and a double digest. Instead of fully methylated adapters, semi-methylated adapters are now used. The bioinformatics pipeline was improved in speed and integrated in the snakemake workflow management system, which now makes the pipeline easy to execute, modular, and parameter settings flexible. We also provide a detailed description of the laboratory protocol, an extensive manual of the bioinformatics pipeline, which is publicly accessible on github (https://github.com/nioo-knaw/epiGBS2) and zenodo (https://doi.org/10.5281/zenodo.3819996), and example output.Competing Interest StatementThe authors have declared no competing interest.",
keywords = "international, Plan_S-Compliant_OA",
author = "Fleur Gawehns and Maarten Postuma and {van Gurp}, {Thomas P.} and Wagemaker, {Niels C. A. M.} and Samar Fatma and {Van Antro}, Morgane and Christa Mateman and Slavica Milanovic-Ivanovic and {van Oers}, Kees and Ivo Grosse and Philippine Vergeer and Verhoeven, {Koen J. F.}",
note = "7051, AnE; Data archiving, no data (software)",
year = "2020",
doi = "10.1101/2020.06.23.137091",
language = "English",
series = "bioRxiv",
publisher = "Cold Spring Harbor Laboratory Press",
address = "United States",
type = "WorkingPaper",
institution = "Cold Spring Harbor Laboratory Press",
}