Evidence for isotopic exchange during metabolism of stable- isotope-labeled formate in a methanogenic sediment

W. De Graaf, T.E. Cappenberg

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    The disappearance of stable-isotope-labeled formate in freshwater sediment from Lake Vechten was studied by gas chromatography-mass spectrometry. Both deuterium- and C-13- labeled formate mere removed from pore water at a high rate (400 +/- 35 [standard deviation] and 1,700 +/- 600 mu M h(-1), respectively), with concomitant formation of nonlabeled formate. The removal rate of labeled and nonlabeled formate amounted to 240 +/- 15 and 400 +/- 40 mu M h(-1), respectively. The formation of nonlabeled formate is explained by isotopic exchange due to the activity of formate dehydrogenase rather than by the turnover of formate. [KEYWORDS: Sulfate-reducing bacteria; marine sediment; acetate; turnover; hydrogen; lake]
    Originele taal-2Engels
    Pagina's (van-tot)3535-3537
    TijdschriftApplied and Environmental Microbiology
    Volume62
    Nummer van het tijdschrift9
    StatusGepubliceerd - 1996

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