TY - JOUR
T1 - Experimental evidence for the impact of phages on mineralization of soil-derived dissolved organic matter under different temperature regimes
AU - Wang, Shuang
AU - Yu, Senxiang
AU - Zhao, Xiaoyan
AU - Zhao, Xiaolei
AU - Mason-Jones, Kyle
AU - Zhu, Zhenke
AU - Redmile-Gordon, Marc
AU - Li, Yong
AU - Chen, Jianping
AU - Kuzyakov, Yakov
AU - Ge, Tida
N1 - Publisher Copyright:
© 2022 Elsevier B.V.
PY - 2022
Y1 - 2022
N2 - Microbial mineralization of dissolved organic matter (DOM) plays an important role in regulating C and nutrient cycling. Viruses are the most abundant biological agents on Earth, but their effect on the density and activity of soil microorganisms and, consequently, on mineralization of DOM under different temperatures remains poorly understood. To assess the impact of viruses on DOM mineralization, we added soil phage concentrate (active vs. inactive phage control) to four DOM extracts containing inoculated microbial communities and incubated them at 18 °C and 23 °C for 32 days. Infection with active phages generally decreased DOM mineralization at day one and showed accelerated DOM mineralization later (especially from day 5 to 15) compared to that with the inactivated phages. Overall, phage infection increased the microbially driven CO2 release. Notably, while higher temperature increased the total CO2 release, the cumulative CO2 release induced by phage infection (difference between active phages and inactivated control) was not affected. However, higher temperatures advanced the response time of the phages but shortening its active period. Our findings suggest that bacterial predation by phages can significantly affect soil DOM mineralization. Therefore, higher temperatures may accelerate host-phage interactions and thus, the duration of C recycling.
AB - Microbial mineralization of dissolved organic matter (DOM) plays an important role in regulating C and nutrient cycling. Viruses are the most abundant biological agents on Earth, but their effect on the density and activity of soil microorganisms and, consequently, on mineralization of DOM under different temperatures remains poorly understood. To assess the impact of viruses on DOM mineralization, we added soil phage concentrate (active vs. inactive phage control) to four DOM extracts containing inoculated microbial communities and incubated them at 18 °C and 23 °C for 32 days. Infection with active phages generally decreased DOM mineralization at day one and showed accelerated DOM mineralization later (especially from day 5 to 15) compared to that with the inactivated phages. Overall, phage infection increased the microbially driven CO2 release. Notably, while higher temperature increased the total CO2 release, the cumulative CO2 release induced by phage infection (difference between active phages and inactivated control) was not affected. However, higher temperatures advanced the response time of the phages but shortening its active period. Our findings suggest that bacterial predation by phages can significantly affect soil DOM mineralization. Therefore, higher temperatures may accelerate host-phage interactions and thus, the duration of C recycling.
KW - Carbon cycle
KW - Dissolved organic carbon
KW - Soil phages
KW - Viral shunt
KW - Warming
U2 - 10.1016/j.scitotenv.2022.157517
DO - 10.1016/j.scitotenv.2022.157517
M3 - Article
C2 - 35872205
AN - SCOPUS:85134794481
SN - 0048-9697
VL - 846
JO - Science of the Total Environment
JF - Science of the Total Environment
M1 - 157517
ER -