Exploration of tissue-specific gene expression patterns underlying timing of breeding in contrasting temperature environments in a song bird

TY - JOUR

T1 - Exploration of tissue-specific gene expression patterns underlying timing of breeding in contrasting temperature environments in a song bird

AU - Laine, Veronika N.

AU - Verhagen, Irene

AU - Mateman, A. Christa

AU - Pijl, Agata

AU - Williams, Tony D.

AU - Gienapp, Phillip

AU - van Oers, Kees

AU - Visser, Marcel E.

N1 - 6804, AnE; Data archiving: data archived in ENA (not NIOO data)

PY - 2019/9/2

Y1 - 2019/9/2

N2 - Seasonal timing of breeding is a life history trait with major fitness consequences but the genetic basis of the physiological mechanism underlying it, and how gene expression is affected by date and temperature, is not well known. In order to study this, we measured patterns of gene expression over different time points in three different tissues of the hypothalamic-pituitary-gonadal-liver axis, and investigated specifically how temperature affects this axis during breeding. We studied female great tits (Parus major) from lines artificially selected for early and late timing of breeding that were housed in two contrasting temperature environments in climate-controlled aviaries. We collected hypothalamus, liver and ovary samples at three different time points (before and after onset of egg-laying). For each tissue, we sequenced whole transcriptomes of 12 pools (n = 3 females) to analyse gene expression.

AB - Seasonal timing of breeding is a life history trait with major fitness consequences but the genetic basis of the physiological mechanism underlying it, and how gene expression is affected by date and temperature, is not well known. In order to study this, we measured patterns of gene expression over different time points in three different tissues of the hypothalamic-pituitary-gonadal-liver axis, and investigated specifically how temperature affects this axis during breeding. We studied female great tits (Parus major) from lines artificially selected for early and late timing of breeding that were housed in two contrasting temperature environments in climate-controlled aviaries. We collected hypothalamus, liver and ovary samples at three different time points (before and after onset of egg-laying). For each tissue, we sequenced whole transcriptomes of 12 pools (n = 3 females) to analyse gene expression.

KW - national

U2 - 10.1186/s12864-019-6043-0

DO - 10.1186/s12864-019-6043-0

M3 - Article

SN - 1471-2164

VL - 20

JO - BMC Genomics

JF - BMC Genomics

IS - 1

ER -