Extracellular matrix hydrogel derived from decellularized tissues enables endodermal organoid culture

Giovanni Giuseppe Giobbe, Claire Crowley, Camilla Luni, Sara Campinoti, Moustafa Khedr, Kai Kretzschmar, Martina Maria De Santis, Elisa Zambaiti, Federica Michielin, Laween Meran, Qianjiang Hu, Gijs van Son, Luca Urbani, Anna Manfredi, Monica Giomo, Simon Eaton, Davide Cacchiarelli, Vivian S W Li, Hans Clevers, Paola BonfantiNicola Elvassore, Paolo De Coppi

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgaveArtikelWetenschappelijkpeer review

Samenvatting

Organoids have extensive therapeutic potential and are increasingly opening up new avenues within regenerative medicine. However, their clinical application is greatly limited by the lack of effective GMP-compliant systems for organoid expansion in culture. Here, we envisage that the use of extracellular matrix (ECM) hydrogels derived from decellularized tissues (DT) can provide an environment capable of directing cell growth. These gels possess the biochemical signature of tissue-specific ECM and have the potential for clinical translation. Gels from decellularized porcine small intestine (SI) mucosa/submucosa enable formation and growth of endoderm-derived human organoids, such as gastric, hepatic, pancreatic, and SI. ECM gels can be used as a tool for direct human organoid derivation, for cell growth with a stable transcriptomic signature, and for in vivo organoid delivery. The development of these ECM-derived hydrogels opens up the potential for human organoids to be used clinically.

Originele taal-2Engels
Pagina's (van-tot)5658
TijdschriftNature Communications
Volume10
Nummer van het tijdschrift1
DOI's
StatusGepubliceerd - 11 dec 2019

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