First DNA Barcoding Survey in Bulgaria Unveiled Huge Diversity of Yeasts in Insects

Roumen Dimitrov; Dilnora Gouliamova; Borislav Guéorguiev; Maudy Smith; Marizeth Groenewald; Teun Boekhout

doi:10.3390/insects15080566

First DNA Barcoding Survey in Bulgaria Unveiled Huge Diversity of Yeasts in Insects

Roumen Dimitrov, Dilnora Gouliamova^*, Borislav Guéorguiev, Maudy Smith, Marizeth Groenewald, Teun Boekhout

^*Bijbehorende auteur voor dit werk

Onderzoeksoutput: Bijdrage aan wetenschappelijk tijdschrift/periodieke uitgave › Artikel › Wetenschappelijk › peer review

Samenvatting

In this study, we conducted a comprehensive survey aimed at assessing the diversity of yeast species inhabiting the guts of various insect species collected mainly from two Bulgarian National Parks, namely, Rila, and Pirin. The insect specimens encompass a broad taxonomic spectrum, including representatives from Coleoptera, Orthoptera, Lepidoptera, Hymenoptera, Dermaptera, Isopoda, and Collembola. Yeast strains were identified with DNA barcoding using the ribosomal markers, specifically, the D1/D2 domains of the ribosomal large subunit (LSU) and the internal transcribed spacers regions ITS 1 + 2 (ITS). The analysis unveiled the presence of 89 ascomycetous and 18 basidiomycetous yeast isolates associated with the insect specimens. Furthermore, our study identified 18 hitherto unknown yeast species.

Originele taal-2	Engels
Artikelnummer	566
Tijdschrift	Insects
Volume	15
Nummer van het tijdschrift	8
DOI's	https://doi.org/10.3390/insects15080566
Status	Gepubliceerd - aug. 2024

Toegang tot document

10.3390/insects15080566

Andere bestanden en links

Link naar publicatie in Scopus

Citeer dit

@article{4619c7d7154243fc8dc3b2b38a406851,

title = "First DNA Barcoding Survey in Bulgaria Unveiled Huge Diversity of Yeasts in Insects",

abstract = "In this study, we conducted a comprehensive survey aimed at assessing the diversity of yeast species inhabiting the guts of various insect species collected mainly from two Bulgarian National Parks, namely, Rila, and Pirin. The insect specimens encompass a broad taxonomic spectrum, including representatives from Coleoptera, Orthoptera, Lepidoptera, Hymenoptera, Dermaptera, Isopoda, and Collembola. Yeast strains were identified with DNA barcoding using the ribosomal markers, specifically, the D1/D2 domains of the ribosomal large subunit (LSU) and the internal transcribed spacers regions ITS 1 + 2 (ITS). The analysis unveiled the presence of 89 ascomycetous and 18 basidiomycetous yeast isolates associated with the insect specimens. Furthermore, our study identified 18 hitherto unknown yeast species.",

keywords = "biodiversity survey, insects, taxonomy, yeasts",

author = "Roumen Dimitrov and Dilnora Gouliamova and Borislav Gu{\'e}orguiev and Maudy Smith and Marizeth Groenewald and Teun Boekhout",

note = "Publisher Copyright: {\textcopyright} 2024 by the authors.",

year = "2024",

month = aug,

doi = "10.3390/insects15080566",

language = "English",

volume = "15",

journal = "Insects",

issn = "2075-4450",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "8",

}

TY - JOUR

T1 - First DNA Barcoding Survey in Bulgaria Unveiled Huge Diversity of Yeasts in Insects

AU - Dimitrov, Roumen

AU - Gouliamova, Dilnora

AU - Guéorguiev, Borislav

AU - Smith, Maudy

AU - Groenewald, Marizeth

AU - Boekhout, Teun

PY - 2024/8

Y1 - 2024/8

N2 - In this study, we conducted a comprehensive survey aimed at assessing the diversity of yeast species inhabiting the guts of various insect species collected mainly from two Bulgarian National Parks, namely, Rila, and Pirin. The insect specimens encompass a broad taxonomic spectrum, including representatives from Coleoptera, Orthoptera, Lepidoptera, Hymenoptera, Dermaptera, Isopoda, and Collembola. Yeast strains were identified with DNA barcoding using the ribosomal markers, specifically, the D1/D2 domains of the ribosomal large subunit (LSU) and the internal transcribed spacers regions ITS 1 + 2 (ITS). The analysis unveiled the presence of 89 ascomycetous and 18 basidiomycetous yeast isolates associated with the insect specimens. Furthermore, our study identified 18 hitherto unknown yeast species.

AB - In this study, we conducted a comprehensive survey aimed at assessing the diversity of yeast species inhabiting the guts of various insect species collected mainly from two Bulgarian National Parks, namely, Rila, and Pirin. The insect specimens encompass a broad taxonomic spectrum, including representatives from Coleoptera, Orthoptera, Lepidoptera, Hymenoptera, Dermaptera, Isopoda, and Collembola. Yeast strains were identified with DNA barcoding using the ribosomal markers, specifically, the D1/D2 domains of the ribosomal large subunit (LSU) and the internal transcribed spacers regions ITS 1 + 2 (ITS). The analysis unveiled the presence of 89 ascomycetous and 18 basidiomycetous yeast isolates associated with the insect specimens. Furthermore, our study identified 18 hitherto unknown yeast species.

KW - biodiversity survey

KW - insects

KW - taxonomy

KW - yeasts

UR - http://www.scopus.com/inward/record.url?scp=85202607894&partnerID=8YFLogxK

U2 - 10.3390/insects15080566

DO - 10.3390/insects15080566

M3 - Article

AN - SCOPUS:85202607894

SN - 2075-4450

VL - 15

JO - Insects

JF - Insects

IS - 8

M1 - 566

ER -